TY - JOUR
T1 - An approach for evaluating the effects of dietary fiber polysaccharides on the human gut microbiome and plasma proteome
AU - Delannoy-Bruno, Omar
AU - Desai, Chandani
AU - Castillo, Juan J.
AU - Couture, Garret
AU - Barve, Ruteja A.
AU - Lombard, Vincent
AU - Henrissat, Bernard
AU - Cheng, Jiye
AU - Han, Nathan
AU - Hayashi, David K.
AU - Meynier, Alexandra
AU - Vinoy, Sophie
AU - Lebrilla, Carlito B.
AU - Marion, Stacey
AU - Heath, Andrew C.
AU - Barratt, Michael J.
AU - Gordon, Jeffrey I.
N1 - Funding Information:
We are indebted to Denise Schmitz for assistance with coordination and oversight of the human studies, including collecting clinical meta-data and fecal samples; Su Deng, Justin Serugo, Kazi Ahsan, Julia Veitinger, Samantha Bale, Jessica Forman, and Sebastian Karlsson for archiving and processing human biospecimens; Marty Meier, Jessica Hoisington-López, and MariaLynn Crosby for fecal microbiome shotgun sequencing; Twyla Juehne, Andrew Lutz, and Jinsheng Yu (Genome Technology Access Center [GTAC] at Washington University in St. Louis) for generating SOMAscan datasets; and Chad Storer and Rich Head (GTAC) for their assistance with CompBio analyses. We also thank Gautier Cesbron Lavau and Monika Okoniewska (Mondelez Global LLC) for preparing the snack prototypes used in the human studies and Laura Kyro for assistance with figure illustrations. This study was supported by the NIH (DK78669 and DK70977), and Mondelez Global, LLC. as part of an academic-industrial collaboration. O.D.-B. received predoctoral stipend support from NIH (R25GM103757, T32GM007067, and T32HL130357). J.I.G. is the recipient of a Thought Leader Award from Agilent Technologies.
Funding Information:
meta-data and fecal samples; Su Deng, Justin Serugo, Kazi Ahsan, Julia Vei-tinger, Samantha Bale, Jessica Forman, and Sebastian Karlsson for archiving and processing human biospecimens; Marty Meier, Jessica Hoisington-López, and MariaLynn Crosby for fecal microbiome shotgun sequencing; Twyla Juehne, Andrew Lutz, and Jinsheng Yu (Genome Technology Access Center [GTAC] at Washington University in St. Louis) for generating SOMAscan datasets; and Chad Storer and Rich Head (GTAC) for their assistance with CompBio analyses. We also thank Gautier Cesbron Lavau and Monika Okoniewska (Mondelµez Global LLC) for preparing the snack prototypes used in the human studies and Laura Kyro for assistance with figure illustrations. This study was supported by the NIH (DK78669 and DK70977), and Mondelµez Global, LLC. as part of an academic-industrial collaboration. O.D.-B. received predoctoral stipend support from NIH (R25GM103757, T32GM007067, and T32HL130357). J.I.G. is the recipient of a Thought Leader Award from Agilent Technologies.
Publisher Copyright:
Copyright © 2022 the Author(s).
PY - 2022/5/17
Y1 - 2022/5/17
N2 - Increases in snack consumption associated with Westernized lifestyles provide an opportunity to introduce nutritious foods into poor diets. We describe two 10-wk-long open label, single group assignment human studies that measured the effects of two snack prototypes containing fiber preparations from two sustainable and scalable sources; the byproducts remaining after isolation of protein from the endosperm of peas and the vesicular pulp remaining after processing oranges for the manufacture of juices. The normal diets of study participants were supplemented with either a pea- or orange fiber-containing snack. We focused our analysis on quantifying the abundances of genes encoding carbohydrate-active enzymes (CAZymes) (glycoside hydrolases and polysaccharide lyases) in the fecal microbiome, mass spectrometric measurements of glycan structures (glycosidic linkages) in feces, plus aptamer-based assessment of levels of 1,300 plasma proteins reflecting a broad range of physiological functions. Computational methods for feature selection identified treatment-discriminatory changes in CAZyme genes that correlated with alterations in levels of fiber-associated glycosidic linkages; these changes in turn correlated with levels of plasma proteins representing diverse biological functions, including transforming growth factor type β/bone morphogenetic protein-mediated fibrosis, vascular endothelial growth factor-related angiogenesis, P38/MAPK-associated immune cell signaling, and obesity-associated hormonal regulators. The approach used represents a way to connect changes in consumer microbiomes produced by specific fiber types with host responses in the context of varying background diets.
AB - Increases in snack consumption associated with Westernized lifestyles provide an opportunity to introduce nutritious foods into poor diets. We describe two 10-wk-long open label, single group assignment human studies that measured the effects of two snack prototypes containing fiber preparations from two sustainable and scalable sources; the byproducts remaining after isolation of protein from the endosperm of peas and the vesicular pulp remaining after processing oranges for the manufacture of juices. The normal diets of study participants were supplemented with either a pea- or orange fiber-containing snack. We focused our analysis on quantifying the abundances of genes encoding carbohydrate-active enzymes (CAZymes) (glycoside hydrolases and polysaccharide lyases) in the fecal microbiome, mass spectrometric measurements of glycan structures (glycosidic linkages) in feces, plus aptamer-based assessment of levels of 1,300 plasma proteins reflecting a broad range of physiological functions. Computational methods for feature selection identified treatment-discriminatory changes in CAZyme genes that correlated with alterations in levels of fiber-associated glycosidic linkages; these changes in turn correlated with levels of plasma proteins representing diverse biological functions, including transforming growth factor type β/bone morphogenetic protein-mediated fibrosis, vascular endothelial growth factor-related angiogenesis, P38/MAPK-associated immune cell signaling, and obesity-associated hormonal regulators. The approach used represents a way to connect changes in consumer microbiomes produced by specific fiber types with host responses in the context of varying background diets.
KW - carbohydrate-active enzymes
KW - fiber-glycan metabolism
KW - gut microbiome-directed foods
KW - microbiome-plasma proteome relationships
UR - http://www.scopus.com/inward/record.url?scp=85129512137&partnerID=8YFLogxK
U2 - 10.1073/pnas.2123411119
DO - 10.1073/pnas.2123411119
M3 - Article
C2 - 35533274
AN - SCOPUS:85129512137
SN - 0027-8424
VL - 119
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 20
M1 - e2123411119
ER -