An altered conformation of nucleosomal core particle in the active chromatin of Physarum polycephalum

Marta Czupryn, Lilianna Solnica, Kazimierz Toczko

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Abstract

We show that nucleosomal particles from transcriptionally active chromatin (MgCl2-soluble fraction) of Physarum polycephalum have lower electrophoretic mobility than those of the bulk chromatin (MgCl2-insoluble fraction). Altered electrophoretic behaviour is observed even at the level of the nucleosomal core, and is shown not to result from its different DNA, RNA or protein content, nor to be a consequence of the process of DNA replication. Analysis of nucelosomes from both fractions on density gradient gels reveals that the slow migration of active nucleosomes is due to increased friction, i.e., more asymmetrical (unfolded) conformation of the nucleosomal core.

Original languageEnglish
Pages (from-to)252-257
Number of pages6
JournalBBA - Gene Structure and Expression
Volume866
Issue number4
DOIs
StatePublished - May 5 1986

Keywords

  • (P. polycephalum)
  • Nucleosome structure
  • Transcriptionally active chromatin

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