TY - JOUR
T1 - An altered conformation of nucleosomal core particle in the active chromatin of Physarum polycephalum
AU - Czupryn, Marta
AU - Solnica, Lilianna
AU - Toczko, Kazimierz
PY - 1986/5/5
Y1 - 1986/5/5
N2 - We show that nucleosomal particles from transcriptionally active chromatin (MgCl2-soluble fraction) of Physarum polycephalum have lower electrophoretic mobility than those of the bulk chromatin (MgCl2-insoluble fraction). Altered electrophoretic behaviour is observed even at the level of the nucleosomal core, and is shown not to result from its different DNA, RNA or protein content, nor to be a consequence of the process of DNA replication. Analysis of nucelosomes from both fractions on density gradient gels reveals that the slow migration of active nucleosomes is due to increased friction, i.e., more asymmetrical (unfolded) conformation of the nucleosomal core.
AB - We show that nucleosomal particles from transcriptionally active chromatin (MgCl2-soluble fraction) of Physarum polycephalum have lower electrophoretic mobility than those of the bulk chromatin (MgCl2-insoluble fraction). Altered electrophoretic behaviour is observed even at the level of the nucleosomal core, and is shown not to result from its different DNA, RNA or protein content, nor to be a consequence of the process of DNA replication. Analysis of nucelosomes from both fractions on density gradient gels reveals that the slow migration of active nucleosomes is due to increased friction, i.e., more asymmetrical (unfolded) conformation of the nucleosomal core.
KW - (P. polycephalum)
KW - Nucleosome structure
KW - Transcriptionally active chromatin
UR - http://www.scopus.com/inward/record.url?scp=0022517454&partnerID=8YFLogxK
U2 - 10.1016/0167-4781(86)90050-3
DO - 10.1016/0167-4781(86)90050-3
M3 - Article
AN - SCOPUS:0022517454
SN - 0167-4781
VL - 866
SP - 252
EP - 257
JO - BBA - Gene Structure and Expression
JF - BBA - Gene Structure and Expression
IS - 4
ER -