An air pressure system for the injection of tracer substances into the brain

D. G. Amaral; J. L. Price

doi:10.1016/0165-0270(83)90107-3

An air pressure system for the injection of tracer substances into the brain

D. G. Amaral, J. L. Price

Department of Neuroscience

Research output: Contribution to journal › Article › peer-review

102 Scopus citations

Abstract

A system for injecting tracers and other substances into the brain through glass micropipettes using mechanically or electromechanically regulated air pressure as the driving force is described. The system has a number of advantages over currently used devices including: accurate ejection of a wide range of volumes (2 nl to several microliters); the capability to inject all currently utilized tracers; and, the direct visualization of the volume of solution injected. This system has proven reliable for a number of tract tracing experiments ranging from the injection of tracers into deep structures of the monkey brain to injecting small quantities of tracers into the brains of fetal rats.

Original language	English
Pages (from-to)	35-43
Number of pages	9
Journal	Journal of Neuroscience Methods
Volume	9
Issue number	1
DOIs	https://doi.org/10.1016/0165-0270(83)90107-3
State	Published - Sep 1983

Keywords

axonal tracers
electromechanical valving system
micropipettes
pressure injection system
tracer injection

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10.1016/0165-0270(83)90107-3

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title = "An air pressure system for the injection of tracer substances into the brain",

abstract = "A system for injecting tracers and other substances into the brain through glass micropipettes using mechanically or electromechanically regulated air pressure as the driving force is described. The system has a number of advantages over currently used devices including: accurate ejection of a wide range of volumes (2 nl to several microliters); the capability to inject all currently utilized tracers; and, the direct visualization of the volume of solution injected. This system has proven reliable for a number of tract tracing experiments ranging from the injection of tracers into deep structures of the monkey brain to injecting small quantities of tracers into the brains of fetal rats.",

keywords = "axonal tracers, electromechanical valving system, micropipettes, pressure injection system, tracer injection",

author = "Amaral, {D. G.} and Price, {J. L.}",

note = "Funding Information: Many of the initial elementos f this systemw ered evelopedb y Dr. Lewis Haberly while hew as a postdoctorafel llowin Dr. Price'sla boratoryW. e gladly acknowledge his contributionsa,n d those of severalo therc olleaguews ith whom this systemh as been discussedT. he circuit illustratedin Fig. 4 was designedb y Dr. Gavin Perry. This work was supportedi n part by NIH Grants NS-09518 to J.L.P. and NS-16980 to W.M. Cowan, by the Clayton Foundationf or Research-California Division and by the McKnight Foundation.",

year = "1983",

month = sep,

doi = "10.1016/0165-0270(83)90107-3",

language = "English",

volume = "9",

pages = "35--43",

journal = "Journal of Neuroscience Methods",

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T1 - An air pressure system for the injection of tracer substances into the brain

AU - Amaral, D. G.

AU - Price, J. L.

N1 - Funding Information: Many of the initial elementos f this systemw ered evelopedb y Dr. Lewis Haberly while hew as a postdoctorafel llowin Dr. Price'sla boratoryW. e gladly acknowledge his contributionsa,n d those of severalo therc olleaguews ith whom this systemh as been discussedT. he circuit illustratedin Fig. 4 was designedb y Dr. Gavin Perry. This work was supportedi n part by NIH Grants NS-09518 to J.L.P. and NS-16980 to W.M. Cowan, by the Clayton Foundationf or Research-California Division and by the McKnight Foundation.

PY - 1983/9

Y1 - 1983/9

N2 - A system for injecting tracers and other substances into the brain through glass micropipettes using mechanically or electromechanically regulated air pressure as the driving force is described. The system has a number of advantages over currently used devices including: accurate ejection of a wide range of volumes (2 nl to several microliters); the capability to inject all currently utilized tracers; and, the direct visualization of the volume of solution injected. This system has proven reliable for a number of tract tracing experiments ranging from the injection of tracers into deep structures of the monkey brain to injecting small quantities of tracers into the brains of fetal rats.

AB - A system for injecting tracers and other substances into the brain through glass micropipettes using mechanically or electromechanically regulated air pressure as the driving force is described. The system has a number of advantages over currently used devices including: accurate ejection of a wide range of volumes (2 nl to several microliters); the capability to inject all currently utilized tracers; and, the direct visualization of the volume of solution injected. This system has proven reliable for a number of tract tracing experiments ranging from the injection of tracers into deep structures of the monkey brain to injecting small quantities of tracers into the brains of fetal rats.

KW - axonal tracers

KW - electromechanical valving system

KW - micropipettes

KW - pressure injection system

KW - tracer injection

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U2 - 10.1016/0165-0270(83)90107-3

DO - 10.1016/0165-0270(83)90107-3

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AN - SCOPUS:0020572793

SN - 0165-0270

VL - 9

SP - 35

EP - 43

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

IS - 1

ER -

An air pressure system for the injection of tracer substances into the brain

Abstract

Keywords

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