@article{f948e648821e48f4afa56a0e42ccb016,
title = "An adenoviral vector expressing human adenovirus 5 and 3 fiber proteins for targeting heterogeneous cell populations",
abstract = "Human adenovirus serotype 5 (HAdV-5) attaches to its primary receptor, the coxsackie and adenovirus receptor (CAR) as the first step of infection. However, CAR expression decreases as tumors progress, thereby diminishing the utility of HAdV-5-based vectors for cancer therapy. In contrast, many aggressive tumor cells highly express CD46, a cellular receptor for HAdV-3. We hypothesized that a mosaic HAdV vector, containing two kinds of fiber proteins, would provide extensive transduction in a heterogeneous population of tumor cells with varying expression levels of HAdV receptors. We therefore generated a fiber-mosaic HAdV vector displaying both a chimeric HAdV-3 fiber and the HAdV-5 fiber protein. We verified the structural integrity of purified viral particles and confirmed that the fiber-mosaic HAdV vector has expanded tropism. We conclude that the use of fiber-mosaic HAdV vectors is a promising approach for transducing a heterogeneous cell population with different expression levels of adenovirus receptors.",
keywords = "Adenovirus, Fiber, Mosaic, Tropism expansion, Tumor",
author = "Miho Murakami and Hideyo Ugai and Minghui Wang and Natalya Belousova and Paul Dent and Fisher, {Paul B.} and Glasgow, {Joel N.} and Maaike Everts and Curiel, {David T.}",
note = "Funding Information: We are grateful to Drs. Jeffrey M. Bergelson (University of Pennsylvania), Victor Krasnykh (The University of Texas), Roger Y. Tsien (University of California at San Diego), Justin C. Roth, and Mr. Anand C. Annan, for providing CHO-CAR cells, unpublished plasmid pVK900, the mCherry construct, an unpublished lentiviral vector, and the unpublished plasmid pcDNA3.1_Ad5T-3SK, respectively. We thank Dr. Zhiying You for statistical analysis. We also thank Dr. Masato Yamamoto for useful advice. We gratefully thank Dr. Terje Dokland and Melissa F. Chimento for technical support with TEM at the High-Resolution Imaging Facility of the University of Alabama at Birmingham. This project was supported by award number P01CA104177 from the National Cancer Institute . The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Cancer Institute and the National Institutes of Health. ",
year = "2010",
month = nov,
day = "25",
doi = "10.1016/j.virol.2010.08.010",
language = "English",
volume = "407",
pages = "196--205",
journal = "Virology",
issn = "0042-6822",
number = "2",
}