An adenoviral platform for selective self-assembly and targeted delivery of nanoparticles

Vaibhav Saini, Dmitri V. Martyshkin, Sergei B. Mirov, Alex Perez, Guy Perkins, Mark H. Ellisman, Victoria D. Towner, Hongju Wu, Larisa Pereboeva, Anton Borovjagin, David T. Curiel, Maaike Everts

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Metallic nanoparticles (NPs) can be used for the diagnosis, imaging, and therapy of tumors and cardiovascular disease. However, targeted delivery of NPs to specific cells remains a major limitation for clinical realization of these potential treatment options. Herein, a novel strategy for the specific coupling of NPs to a targeted adenoviral (Ad) platform to deliver NPs to specific cells is defined. Genetic manipulation of the genetherapy vector is combined with a specific chemical coupling strategy. In particular, a high-affinity interaction between a sequence of six-histidine amino acid residues genetically incorporated into Ad capsid proteins and nickel(II) nitrilotriacetic acid on the surface of gold NPs is employed. The selective self-assembly of gold NPs and Ad vectors into multifunctional platforms does not negatively affect the targeting of Ad to specific cells. This opens the possibility of using Ad vectors for targeted NP delivery, thereby providing a new type of combinatorial approach for the treatment of diseases that involves both nanotechnology and gene therapy.

Original languageEnglish
Pages (from-to)262-269
Number of pages8
JournalSmall
Volume4
Issue number2
DOIs
StatePublished - Feb 2008

Keywords

  • Cell recognition
  • Gene expression
  • Nanoparticles
  • Self-assembly
  • Viruses

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