Amplification of a New Region of DNA in an Unselected Laboratory Stock of L. tarentolae: The T Region

MARIA L. PETRILLO‐PEIXOTO, STEPHEN M. BEVERLEY

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Abstract

A new DNA amplification is described from an isolate of the lizard parasite Leishmania tarentolae. This DNA is present in up to 50 copies in the Trager line of this species and present but not amplified in all other lines tested. This amplification has been named the T amplification (for Tarentolae/Trager). Restriction enzyme digestion and electrophoresis of total DNA reveal amplified fragments totalling 19 kb following staining with ethidium bromide, a finding confirmed by the use of specific hybridization probes. Much of the amplified T DNA occurs as extra‐chromosomal circular molecules. No cross‐hybridization was observed between the T region and other amplified DNA of Leishmania, or the maxicircle of L. tarentolae, nor was resistance to methotrexate, chloroquine or primaquine detected in the T‐amplified line. Combined with our previous results showing H region amplification in 2 other unselected lab stocks, these data demonstrate the prevalence of apparently spontaneous gene amplifications in L. tarentolae.

Original languageEnglish
Pages (from-to)257-261
Number of pages5
JournalThe Journal of Protozoology
Volume36
Issue number3
DOIs
StatePublished - May 1989
Externally publishedYes

Keywords

  • DNA hybridization
  • Ethidium bromide
  • molecular cloning

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