AMPA receptor imaging by infrared scanning near-field optical microscopy

J. Generosi; M. Kropf; G. Margaritondo; H. Hirling; S. Catsicas; K. Johnsson; N. H. Tolk; D. W. Piston; A. Cricenti

doi:10.1002/pssc.200779116

AMPA receptor imaging by infrared scanning near-field optical microscopy

J. Generosi
, M. Kropf
, G. Margaritondo
, H. Hirling
, S. Catsicas
, K. Johnsson
, N. H. Tolk
, D. W. Piston
, A. Cricenti

Research output: Contribution to journal › Conference article › peer-review

1 Scopus citations

Abstract

Infrared scanning near-field optical microscopy is used to detect AMPA-type glutamate receptor clusters in hippocampal neuron cells. AMPA's GluR2-subunits were marked with the fluorophore Alexa488 and high-resolution micrographs were taken at 6.25 μm wavelength, corresponding to the maker's strong infrared absorption. The use of infrared light avoids photobleaching and makes this approach suitable for long-term observations; this technique can be used in general to localize different IR-absorbing molecules over the complex background of other cell components.

Original language	English
Pages (from-to)	2641-2644
Number of pages	4
Journal	Physica Status Solidi (C) Current Topics in Solid State Physics
Volume	5
Issue number	8
DOIs	https://doi.org/10.1002/pssc.200779116
State	Published - 2008
Event	7th International Conference on Optics of Surfaces and Interfaces, OSI-VII - Jackson Hole, WY, United States Duration: Jul 15 2007 → Jul 20 2007

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10.1002/pssc.200779116

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title = "AMPA receptor imaging by infrared scanning near-field optical microscopy",

abstract = "Infrared scanning near-field optical microscopy is used to detect AMPA-type glutamate receptor clusters in hippocampal neuron cells. AMPA's GluR2-subunits were marked with the fluorophore Alexa488 and high-resolution micrographs were taken at 6.25 μm wavelength, corresponding to the maker's strong infrared absorption. The use of infrared light avoids photobleaching and makes this approach suitable for long-term observations; this technique can be used in general to localize different IR-absorbing molecules over the complex background of other cell components.",

author = "J. Generosi and M. Kropf and G. Margaritondo and H. Hirling and S. Catsicas and K. Johnsson and Tolk, \{N. H.\} and Piston, \{D. W.\} and A. Cricenti",

year = "2008",

doi = "10.1002/pssc.200779116",

language = "English",

volume = "5",

pages = "2641--2644",

journal = "Physica Status Solidi (C) Current Topics in Solid State Physics",

issn = "1862-6351",

number = "8",

note = "7th International Conference on Optics of Surfaces and Interfaces, OSI-VII ; Conference date: 15-07-2007 Through 20-07-2007",

}

TY - JOUR

T1 - AMPA receptor imaging by infrared scanning near-field optical microscopy

AU - Generosi, J.

AU - Kropf, M.

AU - Margaritondo, G.

AU - Hirling, H.

AU - Catsicas, S.

AU - Johnsson, K.

AU - Tolk, N. H.

AU - Piston, D. W.

AU - Cricenti, A.

PY - 2008

Y1 - 2008

N2 - Infrared scanning near-field optical microscopy is used to detect AMPA-type glutamate receptor clusters in hippocampal neuron cells. AMPA's GluR2-subunits were marked with the fluorophore Alexa488 and high-resolution micrographs were taken at 6.25 μm wavelength, corresponding to the maker's strong infrared absorption. The use of infrared light avoids photobleaching and makes this approach suitable for long-term observations; this technique can be used in general to localize different IR-absorbing molecules over the complex background of other cell components.

AB - Infrared scanning near-field optical microscopy is used to detect AMPA-type glutamate receptor clusters in hippocampal neuron cells. AMPA's GluR2-subunits were marked with the fluorophore Alexa488 and high-resolution micrographs were taken at 6.25 μm wavelength, corresponding to the maker's strong infrared absorption. The use of infrared light avoids photobleaching and makes this approach suitable for long-term observations; this technique can be used in general to localize different IR-absorbing molecules over the complex background of other cell components.

UR - https://www.scopus.com/pages/publications/77951099883

U2 - 10.1002/pssc.200779116

DO - 10.1002/pssc.200779116

M3 - Conference article

AN - SCOPUS:77951099883

SN - 1862-6351

VL - 5

SP - 2641

EP - 2644

JO - Physica Status Solidi (C) Current Topics in Solid State Physics

JF - Physica Status Solidi (C) Current Topics in Solid State Physics

IS - 8

T2 - 7th International Conference on Optics of Surfaces and Interfaces, OSI-VII

Y2 - 15 July 2007 through 20 July 2007

ER -

AMPA receptor imaging by infrared scanning near-field optical microscopy

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