AMPA receptor imaging by infrared scanning near-field optical microscopy

J. Generosi, M. Kropf, G. Margaritondo, H. Hirling, S. Catsicas, K. Johnsson, N. H. Tolk, D. W. Piston, A. Cricenti

Research output: Contribution to journalConference articlepeer-review

1 Scopus citations


Infrared scanning near-field optical microscopy is used to detect AMPA-type glutamate receptor clusters in hippocampal neuron cells. AMPA's GluR2-subunits were marked with the fluorophore Alexa488 and high-resolution micrographs were taken at 6.25 μm wavelength, corresponding to the maker's strong infrared absorption. The use of infrared light avoids photobleaching and makes this approach suitable for long-term observations; this technique can be used in general to localize different IR-absorbing molecules over the complex background of other cell components.

Original languageEnglish
Pages (from-to)2641-2644
Number of pages4
JournalPhysica Status Solidi (C) Current Topics in Solid State Physics
Issue number8
StatePublished - 2008
Event7th International Conference on Optics of Surfaces and Interfaces, OSI-VII - Jackson Hole, WY, United States
Duration: Jul 15 2007Jul 20 2007


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