TY - JOUR
T1 - AMPA receptor imaging by infrared scanning near-field optical microscopy
AU - Generosi, J.
AU - Kropf, M.
AU - Margaritondo, G.
AU - Hirling, H.
AU - Catsicas, S.
AU - Johnsson, K.
AU - Tolk, N. H.
AU - Piston, D. W.
AU - Cricenti, A.
PY - 2008
Y1 - 2008
N2 - Infrared scanning near-field optical microscopy is used to detect AMPA-type glutamate receptor clusters in hippocampal neuron cells. AMPA's GluR2-subunits were marked with the fluorophore Alexa488 and high-resolution micrographs were taken at 6.25 μm wavelength, corresponding to the maker's strong infrared absorption. The use of infrared light avoids photobleaching and makes this approach suitable for long-term observations; this technique can be used in general to localize different IR-absorbing molecules over the complex background of other cell components.
AB - Infrared scanning near-field optical microscopy is used to detect AMPA-type glutamate receptor clusters in hippocampal neuron cells. AMPA's GluR2-subunits were marked with the fluorophore Alexa488 and high-resolution micrographs were taken at 6.25 μm wavelength, corresponding to the maker's strong infrared absorption. The use of infrared light avoids photobleaching and makes this approach suitable for long-term observations; this technique can be used in general to localize different IR-absorbing molecules over the complex background of other cell components.
UR - http://www.scopus.com/inward/record.url?scp=77951099883&partnerID=8YFLogxK
U2 - 10.1002/pssc.200779116
DO - 10.1002/pssc.200779116
M3 - Conference article
AN - SCOPUS:77951099883
SN - 1862-6351
VL - 5
SP - 2641
EP - 2644
JO - Physica Status Solidi (C) Current Topics in Solid State Physics
JF - Physica Status Solidi (C) Current Topics in Solid State Physics
IS - 8
T2 - 7th International Conference on Optics of Surfaces and Interfaces, OSI-VII
Y2 - 15 July 2007 through 20 July 2007
ER -