TY - JOUR
T1 - Amniotic fluid stem cell migration after intraperitoneal injection in pup rats
T2 - Implication for therapy
AU - Ghionzoli, Marco
AU - Cananzi, Mara
AU - Zani, Augusto
AU - Rossi, Carlo Alberto
AU - Fascetti Leon, Francesco
AU - Pierro, Agostino
AU - Eaton, Simon
AU - De Coppi, Paolo
PY - 2010/1
Y1 - 2010/1
N2 - Purpose: Despite being commonly used in clinical practice, the intraperitoneal (i.p.) route has been rarely used for cell delivery. We evaluated the capacity of amniotic fluid stem (AFS) cells, administered i.p., to diffuse systemically and to integrate into tissues of healthy newborn rats. Methods: AFS cells were obtained from pregnant GFP + Sprague-Dawley rats by c-kit selection. Wild-type Sprague-Dawley newborn rats were divided into two groups receiving i.p.: (1) 2 × 106 AFS cells (n = 12); (2) of phosphate buffer saline (PBS) (n = 2) at 24 and 48 h after birth. Animals were either killed at 96 h of life, and organs collected for gfp amplification, or at 3 weeks of life and tissues isolated for green fluorescence protein (GFP) immunofluorescence. Results: No adverse effects were observed after i.p. injection of PBS or AFS cells. Gfp was amplified in at least one organ in all rats injected with AFS cells except one (11/12). The intestine was the organ found most frequently positive (67%) followed by liver (25%), spleen (16%), heart (16%), lungs (16%), femur (8%) and brain (0%). Immunohistochemistry confirmed PCR results. Conclusion: In the short term, the i.p. administration of AFS cells, is a safe procedure and allows their migration, homing and integration into various organs of healthy newborn rats.
AB - Purpose: Despite being commonly used in clinical practice, the intraperitoneal (i.p.) route has been rarely used for cell delivery. We evaluated the capacity of amniotic fluid stem (AFS) cells, administered i.p., to diffuse systemically and to integrate into tissues of healthy newborn rats. Methods: AFS cells were obtained from pregnant GFP + Sprague-Dawley rats by c-kit selection. Wild-type Sprague-Dawley newborn rats were divided into two groups receiving i.p.: (1) 2 × 106 AFS cells (n = 12); (2) of phosphate buffer saline (PBS) (n = 2) at 24 and 48 h after birth. Animals were either killed at 96 h of life, and organs collected for gfp amplification, or at 3 weeks of life and tissues isolated for green fluorescence protein (GFP) immunofluorescence. Results: No adverse effects were observed after i.p. injection of PBS or AFS cells. Gfp was amplified in at least one organ in all rats injected with AFS cells except one (11/12). The intestine was the organ found most frequently positive (67%) followed by liver (25%), spleen (16%), heart (16%), lungs (16%), femur (8%) and brain (0%). Immunohistochemistry confirmed PCR results. Conclusion: In the short term, the i.p. administration of AFS cells, is a safe procedure and allows their migration, homing and integration into various organs of healthy newborn rats.
KW - Amniotic fluid stem cell
KW - Cell therapy
KW - Intraperitoneal injection
KW - Pup rat
UR - http://www.scopus.com/inward/record.url?scp=77649191720&partnerID=8YFLogxK
U2 - 10.1007/s00383-009-2504-x
DO - 10.1007/s00383-009-2504-x
M3 - Article
C2 - 19855985
AN - SCOPUS:77649191720
SN - 0179-0358
VL - 26
SP - 79
EP - 84
JO - Pediatric Surgery International
JF - Pediatric Surgery International
IS - 1
ER -