The complete amino acid sequence of porcine heart fumarase (EC 22.214.171.124) has been determined from peptides produced by cyanogen bromide, endoproteinase Arg-C, S. aureus V8 protease, and trypsin. The enzyme is a tetramer of identical subunits with Mr = 50,015 and composed of 466 amino acid residues. Porcine heart fumarase displays 96% identity to human liver fumarase. Prediction of the secondary structural elements of porcine fumarase indicate that the enzyme contains a large amount of alpha helix with very little beta structure.
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - May 31 1988|