Amino acid sequence of porcine heart fumarase

James C. Sacchettini, Mark W. Frazier, David C. Chiara, Leonard J. Banaszak, Gregory A. Grant

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


The complete amino acid sequence of porcine heart fumarase (EC has been determined from peptides produced by cyanogen bromide, endoproteinase Arg-C, S. aureus V8 protease, and trypsin. The enzyme is a tetramer of identical subunits with Mr = 50,015 and composed of 466 amino acid residues. Porcine heart fumarase displays 96% identity to human liver fumarase. Prediction of the secondary structural elements of porcine fumarase indicate that the enzyme contains a large amount of alpha helix with very little beta structure.

Original languageEnglish
Pages (from-to)435-440
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - May 31 1988


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