TY - JOUR
T1 - Amino acid sequence of porcine heart fumarase
AU - Sacchettini, James C.
AU - Frazier, Mark W.
AU - Chiara, David C.
AU - Banaszak, Leonard J.
AU - Grant, Gregory A.
N1 - Funding Information:
This work was supported in part by NSF grant DNB 8706274. J. C. S. is an Americau Heart Association, Missouri Affiliate, Fellow. We thank Thomas Meininger, Deborah Foley, Ella Jones, and Eric Burr for their excellent technical assistance.
PY - 1988/5/31
Y1 - 1988/5/31
N2 - The complete amino acid sequence of porcine heart fumarase (EC 4.2.1.2) has been determined from peptides produced by cyanogen bromide, endoproteinase Arg-C, S. aureus V8 protease, and trypsin. The enzyme is a tetramer of identical subunits with Mr = 50,015 and composed of 466 amino acid residues. Porcine heart fumarase displays 96% identity to human liver fumarase. Prediction of the secondary structural elements of porcine fumarase indicate that the enzyme contains a large amount of alpha helix with very little beta structure.
AB - The complete amino acid sequence of porcine heart fumarase (EC 4.2.1.2) has been determined from peptides produced by cyanogen bromide, endoproteinase Arg-C, S. aureus V8 protease, and trypsin. The enzyme is a tetramer of identical subunits with Mr = 50,015 and composed of 466 amino acid residues. Porcine heart fumarase displays 96% identity to human liver fumarase. Prediction of the secondary structural elements of porcine fumarase indicate that the enzyme contains a large amount of alpha helix with very little beta structure.
UR - http://www.scopus.com/inward/record.url?scp=0023912630&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(88)81243-9
DO - 10.1016/S0006-291X(88)81243-9
M3 - Article
C2 - 3377794
AN - SCOPUS:0023912630
VL - 153
SP - 435
EP - 440
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -