Amino Acid Sequence of κ-Flavitoxin: Establishment of a New Family of Snake Venom Neurotoxins

Gregory A. Grant; Mark W. Frazier; Vincent A. Chiappinelli

doi:10.1021/bi00410a041

Amino Acid Sequence of κ-Flavitoxin: Establishment of a New Family of Snake Venom Neurotoxins

Gregory A. Grant, Mark W. Frazier, Vincent A. Chiappinelli

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38 Scopus citations

Abstract

The complete amino acid sequence of κ-flavitoxin, a neurotoxin isolated from the venom of Bungarus flaviceps, has been determined by automated Edman analysis of the intact protein and of peptides derived from digests with trypsin and chymotrypsin.κ-Flavitoxin consists of a single 66-residue polypeptide chain which is completely devoid of methionine. the amino acid sequence of κ-flavitoxin demonstrates that although the toxin is related to the α-neurotoxin family, it displays a much higher degree of homology with κ-bungarotoxin. the conserved structural features of the K-neurotoxins and their pharmacological profiles, which are distinct from those of all known α-neurotoxins, provide evidence for a new, structurally and functionally unique family of snake venom neurotoxins.

Original language	English
Pages (from-to)	3794-3798
Number of pages	5
Journal	Biochemistry
Volume	27
Issue number	10
DOIs	https://doi.org/10.1021/bi00410a041
State	Published - May 1 1988

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abstract = "The complete amino acid sequence of κ-flavitoxin, a neurotoxin isolated from the venom of Bungarus flaviceps, has been determined by automated Edman analysis of the intact protein and of peptides derived from digests with trypsin and chymotrypsin.κ-Flavitoxin consists of a single 66-residue polypeptide chain which is completely devoid of methionine. the amino acid sequence of κ-flavitoxin demonstrates that although the toxin is related to the α-neurotoxin family, it displays a much higher degree of homology with κ-bungarotoxin. the conserved structural features of the K-neurotoxins and their pharmacological profiles, which are distinct from those of all known α-neurotoxins, provide evidence for a new, structurally and functionally unique family of snake venom neurotoxins.",

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T1 - Amino Acid Sequence of κ-Flavitoxin

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AU - Chiappinelli, Vincent A.

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N2 - The complete amino acid sequence of κ-flavitoxin, a neurotoxin isolated from the venom of Bungarus flaviceps, has been determined by automated Edman analysis of the intact protein and of peptides derived from digests with trypsin and chymotrypsin.κ-Flavitoxin consists of a single 66-residue polypeptide chain which is completely devoid of methionine. the amino acid sequence of κ-flavitoxin demonstrates that although the toxin is related to the α-neurotoxin family, it displays a much higher degree of homology with κ-bungarotoxin. the conserved structural features of the K-neurotoxins and their pharmacological profiles, which are distinct from those of all known α-neurotoxins, provide evidence for a new, structurally and functionally unique family of snake venom neurotoxins.

AB - The complete amino acid sequence of κ-flavitoxin, a neurotoxin isolated from the venom of Bungarus flaviceps, has been determined by automated Edman analysis of the intact protein and of peptides derived from digests with trypsin and chymotrypsin.κ-Flavitoxin consists of a single 66-residue polypeptide chain which is completely devoid of methionine. the amino acid sequence of κ-flavitoxin demonstrates that although the toxin is related to the α-neurotoxin family, it displays a much higher degree of homology with κ-bungarotoxin. the conserved structural features of the K-neurotoxins and their pharmacological profiles, which are distinct from those of all known α-neurotoxins, provide evidence for a new, structurally and functionally unique family of snake venom neurotoxins.

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Amino Acid Sequence of κ-Flavitoxin: Establishment of a New Family of Snake Venom Neurotoxins

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