Amine-reactive neutron-encoded labels for highly plexed proteomic quantitation

Alexander S. Hebert, Anna E. Merrill, Jonathan A. Stefely, Derek J. Bailey, Craig D. Wenger, Michael S. Westphall, David J. Pagliarini, Joshua J. Coon

Research output: Contribution to journalArticlepeer-review

50 Scopus citations


We describe a novel amine-reactive chemical label that exploits differential neutron-binding energy between 13C and 15N isotopes. These neutron-encoded (NeuCode) chemical labels enable up to 12-plex MS1-based protein quantification. Each structurally identical, but isotopically unique, tag is encoded with a 12.6-mDa mass difference- relative to its nearest neighbor-so that peptides bearing these NeuCode signatures do not increase spectral complexity and are detected only upon analysis with very high mass-resolving powers. We demonstrate that the method provides quantitative performance that is comparable to both metabolic labeling and isobaric tagging while combining the benefits of both strategies. Finally, we employ the tags to characterize the proteome of Saccharomyces cerevisiae during the diauxic shift, a metabolic transition from fermentation to aerobic respiration.c.

Original languageEnglish
Pages (from-to)3360-3369
Number of pages10
JournalMolecular and Cellular Proteomics
Issue number11
StatePublished - Nov 2013


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