Alternative splicing of the human diacylglycerol kinase ζ gene in muscle

Li Ding, Michaeline Bunting, Matthew K. Topham, Thomas M. McIntyre, Guy A. Zimmerman, Stephen M. Prescott

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

Diacylglycerol can function as a second messenger, and one mechanism for the attenuation of this signal is its conversion to phosphatidic acid, which is catalyzed by diacylglycerol kinase (DGK). We screened a cDNA library from human skeletal muscle and isolated two DGKζ cDNAs that differed from the 3.5-kb clone originally identified in endothelial cells. One transcript, which was 3.4 kb long, was shown to be nonfunctional; it had a 77-bp deletion that included the translation initiation site. The other was 4.1 kb long with a unique 5' sequence of 853 bp. We also isolated a genomic clone of DGKζ and determined its organization and location; it contains 32 exons, spans approximately 50 kb of genomic sequence, and maps to chromosome 11p11.2. The protein encoded by the 4.1-kb transcript contains two cysteine-rich regions, a catalytic domain, and ankyrin repeats like the endothelial form of DGKζ, as well as a unique N-terminal domain. The coding sequence was shown to be derived from alternative splicing of the DGKζ gene. In cells transfected with the 4.1-kb clone, we detected a 130-kDa protein with an antibody to DGKζ and demonstrated that it was localized predominantly in the nucleus. We conclude that alternative splicing generates tissue-specific variants of DGKζ that share some properties but may have unique ones as well.

Original languageEnglish
Pages (from-to)5519-5524
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number11
DOIs
StatePublished - May 27 1997

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