Alternative expression analysis by RNA sequencing

Malachi Griffith, Obi L. Griffith, Jill Mwenifumbo, Rodrigo Goya, A. Sorana Morrissy, Ryan D. Morin, Richard Corbett, Michelle J. Tang, Ying Chen Hou, Trevor J. Pugh, Gordon Robertson, Suganthi Chittaranjan, Adrian Ally, Jennifer K. Asano, Susanna Y. Chan, Haiyan I. Li, Helen McDonald, Kevin Teague, Yongjun Zhao, Thomas ZengAllen Delaney, Martin Hirst, Gregg B. Morin, Steven J.M. Jones, Isabella T. Tai, Marco A. Marra

Research output: Contribution to journalArticle

192 Scopus citations

Abstract

In alternative expression analysis by sequencing (ALEXA-seq), we developed a method to analyze massively parallel RNA sequence data to catalog transcripts and assess differential and alternative expression of known and predicted mRNA isoforms in cells and tissues. As proof of principle, we used the approach to compare fluorouracil-resistant and-nonresistant human colorectal cancer cell lines. We assessed the sensitivity and specificity of the approach by comparison to exon tiling and splicing microarrays and validated the results with reverse transcription-PCR, quantitative PCR and Sanger sequencing. We observed global disruption of splicing in fluorouracil-resistant cells characterized by expression of new mRNA isoforms resulting from exon skipping, alternative splice site usage and intron retention. Alternative expression annotation databases, source code, a data viewer and other resources to facilitate analysis are available at http://www.alexaplatform.org/alexa-seq/.

Original languageEnglish
Pages (from-to)843-847
Number of pages5
JournalNature Methods
Volume7
Issue number10
DOIs
StatePublished - Oct 1 2010
Externally publishedYes

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    Griffith, M., Griffith, O. L., Mwenifumbo, J., Goya, R., Morrissy, A. S., Morin, R. D., Corbett, R., Tang, M. J., Hou, Y. C., Pugh, T. J., Robertson, G., Chittaranjan, S., Ally, A., Asano, J. K., Chan, S. Y., Li, H. I., McDonald, H., Teague, K., Zhao, Y., ... Marra, M. A. (2010). Alternative expression analysis by RNA sequencing. Nature Methods, 7(10), 843-847. https://doi.org/10.1038/nmeth.1503