TY - JOUR
T1 - Alterations in Spliced and Unspliced HIV-1-Specific RNA Detection in Peripheral Blood Mononuclear Cells of Individuals with Varying CD4-Positive Lymphocyte Counts
AU - Arens, Max
AU - Joseph, Theresa
AU - Nag, Sutapa
AU - Miller, J. Philip
AU - Powderly, William G.
AU - Ratner, Lee
PY - 1993/12
Y1 - 1993/12
N2 - Reverse transcriptase-polymerase chain amplification reactions (RT-PCR) were used to identify transcripts for HIV-1 structural and regulatory proteins in peripheral blood mononuclear cells of a cohort of 48 patients. At least one set of PCR primers was capable of detecting HIV-1 transcripts in 94% of patients. Unspliced gag-pol transcripts were detected with gag or pol primer sets in 60 and 63% of samples, respectively. A significant inverse correlation was noted between transcript identification with the gag primer set and the number of CD4-positive lymphocytes in the blood sample and the clinical stage of infection. Single-spliced env transcripts were identified in 44% of individuals. Multiple-spliced tat or nef transcripts were detected in 6.2 and 53% of individuals, respectively. These findings indicate that viral transcripts are expressed throughout the course of HIV-1 infection.
AB - Reverse transcriptase-polymerase chain amplification reactions (RT-PCR) were used to identify transcripts for HIV-1 structural and regulatory proteins in peripheral blood mononuclear cells of a cohort of 48 patients. At least one set of PCR primers was capable of detecting HIV-1 transcripts in 94% of patients. Unspliced gag-pol transcripts were detected with gag or pol primer sets in 60 and 63% of samples, respectively. A significant inverse correlation was noted between transcript identification with the gag primer set and the number of CD4-positive lymphocytes in the blood sample and the clinical stage of infection. Single-spliced env transcripts were identified in 44% of individuals. Multiple-spliced tat or nef transcripts were detected in 6.2 and 53% of individuals, respectively. These findings indicate that viral transcripts are expressed throughout the course of HIV-1 infection.
UR - http://www.scopus.com/inward/record.url?scp=0027787919&partnerID=8YFLogxK
U2 - 10.1089/aid.1993.9.1257
DO - 10.1089/aid.1993.9.1257
M3 - Article
C2 - 7908212
AN - SCOPUS:0027787919
SN - 0889-2229
VL - 9
SP - 1257
EP - 1263
JO - AIDS research and human retroviruses
JF - AIDS research and human retroviruses
IS - 12
ER -