TY - JOUR
T1 - Alterations in patient plasma microRNA expression profiles following resection of metastatic melanoma
AU - Latchana, Nicholas
AU - DiVincenzo, Mallory J.
AU - Regan, Kelly
AU - Abrams, Zachary
AU - Zhang, Xiaoli
AU - Jacob, Naduparambil K.
AU - Gru, Alejandro A.
AU - Fadda, Paolo
AU - Markowitz, Joseph
AU - Howard, J. Harrison
AU - Carson, William E.
N1 - Funding Information:
NIH Grants P01 CA095426 (to MA Caligiuri), P30 CA16058 (to MA Caligiuri), T32 CA090223 (to WE Carson), T32 CA009338 (to MA Caligiuri), K24 CA093670 (to WE Carson), NLM grant T15LM011270 to Z Abrams.
Publisher Copyright:
© 2018 Wiley Periodicals, Inc.
PY - 2018/9/1
Y1 - 2018/9/1
N2 - Background and Objectives: MicroRNAs (miRs) are noncoding RNAs that regulate protein translation and melanoma progression. Changes in plasma miR expression following surgical resection of metastatic melanoma are under-investigated. We hypothesize differences in miR expression exist following complete surgical resection of metastatic melanoma. Methods: Blood collection pre- and post-surgical resection was performed in six individuals with solitary melanoma metastases. miR expression in extracted RNA was quantified using the NanoString nCounter Digital Analyzer. Results: Pre- and post-surgical plasma samples contained 216 miRs with expression above baseline. Comparison of postsurgical to preresection samples revealed differential expression of 25 miRs: miR-let-7a, miR-let7g, miR-15a, miR-16, miR-22, miR-30b, miR-126, miR-140, miR-145, miR-148a, miR-150-5p, miR-191, miR-378i, miR-449c, miR-494, miR-513b, miR-548aa, miR-571, miR-587, miR-891b, miR-1260a, miR 1268a, miR-1976, miR-4268, miR-4454 (P < 0.05). Utilizing P < 0.0046 as a cutoff to control for one false positive among the 216 miRs revealed that postsurgical melanoma plasma samples had upregulation of miR-1260a (P = 0.0007) and downregulation of miR-150-5p (P = 0.0026) relative to pre-surgical samples. Conclusions: Differential expression of miR-150-5p and miR-1260a is present in plasma following surgical resection of metastatic melanoma in this small sample (n = 6) of melanoma patients. Therefore, further investigation of these plasma miRs as noninvasive biomarkers for melanoma is warranted.
AB - Background and Objectives: MicroRNAs (miRs) are noncoding RNAs that regulate protein translation and melanoma progression. Changes in plasma miR expression following surgical resection of metastatic melanoma are under-investigated. We hypothesize differences in miR expression exist following complete surgical resection of metastatic melanoma. Methods: Blood collection pre- and post-surgical resection was performed in six individuals with solitary melanoma metastases. miR expression in extracted RNA was quantified using the NanoString nCounter Digital Analyzer. Results: Pre- and post-surgical plasma samples contained 216 miRs with expression above baseline. Comparison of postsurgical to preresection samples revealed differential expression of 25 miRs: miR-let-7a, miR-let7g, miR-15a, miR-16, miR-22, miR-30b, miR-126, miR-140, miR-145, miR-148a, miR-150-5p, miR-191, miR-378i, miR-449c, miR-494, miR-513b, miR-548aa, miR-571, miR-587, miR-891b, miR-1260a, miR 1268a, miR-1976, miR-4268, miR-4454 (P < 0.05). Utilizing P < 0.0046 as a cutoff to control for one false positive among the 216 miRs revealed that postsurgical melanoma plasma samples had upregulation of miR-1260a (P = 0.0007) and downregulation of miR-150-5p (P = 0.0026) relative to pre-surgical samples. Conclusions: Differential expression of miR-150-5p and miR-1260a is present in plasma following surgical resection of metastatic melanoma in this small sample (n = 6) of melanoma patients. Therefore, further investigation of these plasma miRs as noninvasive biomarkers for melanoma is warranted.
KW - NanoString
KW - miR
KW - miR-1260a
KW - miR-150-5p
KW - miRNA
UR - http://www.scopus.com/inward/record.url?scp=85052625785&partnerID=8YFLogxK
U2 - 10.1002/jso.25163
DO - 10.1002/jso.25163
M3 - Article
C2 - 30132912
AN - SCOPUS:85052625785
VL - 118
SP - 501
EP - 509
JO - Journal of Surgical Oncology
JF - Journal of Surgical Oncology
SN - 0022-4790
IS - 3
ER -