Alteration of Golgi structure in senescent cells and its regulation by a G protein γ subunit

Joon Ho Cho, Deepak Kumar Saini, W. K.Ajith Karunarathne, Vani Kalyanaraman, N. Gautam

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Cellular senescence is a process wherein proliferating cells undergo permanent cell cycle arrest while remaining viable. Senescence results in enhanced secretion of proteins that promote cancer and inflammation. We report here that the structure of the Golgi complex which regulates secretion is altered in senescent cells. In cells where senescence is achieved by replicative exhaustion or in cells wherein senescence has been induced with BrdU treatment dependent stress, the Golgi complex is dispersed. The expression of a G protein γ subunit, γ11, capable of translocation from the plasma membrane to the Golgi complex on receptor activation increases with senescence. Knockdown of γ11 or overexpression of a dominant negative γ3 subunit inhibits Golgi dispersal induced by senescence. Overall these results suggest that in cellular senescence an upregulated G protein gamma subunit mediates alterations in the structure of the Golgi.

Original languageEnglish
Pages (from-to)785-793
Number of pages9
JournalCellular Signalling
Volume23
Issue number5
DOIs
StatePublished - May 2011

Keywords

  • Betagamma subunits
  • Cell senescence
  • G protein
  • Translocation

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