Alphavirus-based expression vectors: Strategies and applications

Ilya Frolov, Thomas A. Hoffman, Béla M. Prágai, Sergey A. Dryga, Henry V. Huang, Sondra Schlesinger, Charles M. Rice

Research output: Contribution to journalArticle

164 Scopus citations

Abstract

Alphaviruses are positive-strand RNA viruses that can mediate efficent cytoplasmic gene expression in insect and vertebrate cells. Through recombinant DNA technology, the alphavirus RNA replication machinery has been engineered for high-level expression of heterologous RNAs and proteins. Amplification of replication-competent alphavirus RNAs (replicons) can be initiated by RNA or DNA transfection and a variety of packaging systems have been developed for producing high titers of infectious viral particles. Although normally cytocidal for vertebrate cells, variants with adaptive mutations allowing noncytopathic replication have been isolated from persistently infected cultures or selected using a dominant selectable marker. Such mutations have been mapped and used to create new alphavirus vectors for noncytopathic gene expression in mammalian cells. These vectors allow long-term expression at moderate levels and complement previous vectors designed for short-term high-level expression. Besides their use for a growing number of basic research applications, recombinant alphavirus RNA replicons may also facilitate genetic vaccination and transient gene therapy.

Original languageEnglish
Pages (from-to)11371-11377
Number of pages7
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number21
DOIs
StatePublished - Oct 15 1996

Keywords

  • RNA replicon
  • gene therapy
  • nucleic acid vaccines
  • protein production

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