Allogeneic peripheral blood stem cell transplantation following CD34+ enrichment by density gradient separation

R. Vij, R. Brown, S. Shenoy, J. S. Haug, D. Kaesberg, D. Adkins, L. T. Goodnough, H. Khoury, J. DiPersio

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

GVHD is a significant cause of morbidity and mortality following allogeneic peripheral blood stem cell transplantation (AlloPBSC). CD34+ cell selection could reduce GVHD by negative selection of T cells. In an attempt to reduce the T cell content of alloPBSC we carried out a trial in which 11 patients with hematologic malignancies received alloPBSC from HLA-matched siblings following density gradient separation using an isotonic colloidal silica solution (BDS 60; Dendreon Corporation). Cyclosporine and methylprednisone were used for GVHD prophylaxis. The mean yield of CD34+ cells was 69 ± 15.6% with a purity of 2.9 ± 1.7%. The mean number of CD3+ cells infused was 1.0 ± 1.2 x 107/kg, representing a 1.3 log depletion. A high risk of acute GVHD was observed: grade II-IV in 7/11 (64%) and grade III-IV GVHD in 5/11 (45%) patients. Nine of the 11 (82%) patients died with a median survival of 68 days. Cytokine expression in PBSC was compared pre and post processing. Interferon-gamma was detected only following density gradient separation while IL-8 expression increased 3- to 6-fold post processing. Therefore, processing with this device may augment production of pro-inflammatory cytokines.

Original languageEnglish
Pages (from-to)1223-1228
Number of pages6
JournalBone Marrow Transplantation
Volume25
Issue number12
DOIs
StatePublished - 2000

Keywords

  • Allogeneic transplantation
  • CD34 selection
  • Cytokines
  • Dendritic cells
  • GVHD

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