Allelic Loss on Chromosome 17 in Ductal Carcinoma in Situ of the Breast

Diane M. Radford, Keri Fair, Alastair M. Thompson, Jon H. Ritter, Matthew Holt, Todd Steinbrueck, Mairi Wallace, Samuel A. Wells, Helen R. Donis-Keller

Research output: Contribution to journalArticlepeer-review

74 Scopus citations

Abstract

Multiple tumor suppressor genes are implicated in the oncogenesis and progression of invasive carcinoma of the breast To investigate the chronology of genetic changes we studied loss of heterozygosity on chromosome 17 in ductal carcinoma in situ, a preinvasive breast cancer. A microdissection technique was used to separate tumor from normal stromal cells prior to DNA extraction and loss of heterozygosity was assayed mainly using simple sequence repeat polymorphism markers and the polymerase chain reaction. Loss of heterozygosity on 17p was observed in 8 of 28 tumors (29%) when compared with normal control DNA, whereas no loss was seen on 17q, suggesting that at least one locus on 17p is involved early in the development of breast cancer.

Original languageEnglish
Pages (from-to)2947-2949
Number of pages3
JournalCancer research
Volume53
Issue number13
StatePublished - Aug 1993

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