Alkaline phosphatase reporter transposon for identification of genes encoding secreted proteins in gram-positive microorganisms

Carmela M. Gibson, Michael G. Caparon

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

We describe the construction of TnFuZ, a genetic tool for the discovery and mutagenesis of proteins exported from gram-positive bacteria. This tool combines a transposable element (Tn4001) of broad host range in gram-positive bacteria and an alkaline phosphatase gene (phoZ) derived from a gram-positive bacterium that has been modified by removal of the region encoding its export signal. Mutagenesis of Streptococcus pyogenes with TnFuZ ("FuZ" stands for fusions to phoZ) identified genes encoding secreted proteins whose expression was enhanced during growth in an aerobic environment. Thus, TnFuZ should be valuable for analysis of protein secretion, gene regulation, and virulence in gram-positive bacteria.

Original languageEnglish
Pages (from-to)928-932
Number of pages5
JournalApplied and Environmental Microbiology
Volume68
Issue number2
DOIs
StatePublished - 2002

Fingerprint

Dive into the research topics of 'Alkaline phosphatase reporter transposon for identification of genes encoding secreted proteins in gram-positive microorganisms'. Together they form a unique fingerprint.

Cite this