TY - JOUR
T1 - Airy-beam holographic sonogenetics for advancing neuromodulation precision and flexibility
AU - Hu, Zhongtao
AU - Yang, Yaoheng
AU - Yang, Leqi
AU - Gong, Yan
AU - Chukwu, Chinwendu
AU - Ye, Dezhuang
AU - Yue, Yimei
AU - Yuan, Jinyun
AU - Kravitz, Alexxai V.
AU - Chen, Hong
N1 - Publisher Copyright:
© 2024 the Author(s). Published by PNAS.
PY - 2024/6/1
Y1 - 2024/6/1
N2 - Advancing our understanding of brain function and developing treatments for neurological diseases hinge on the ability to modulate neuronal groups in specific brain areas without invasive techniques. Here, we introduce Airy-beam holographic sonogenetics (AhSonogenetics) as an implant-free, cell type–specific, spatially precise, and flexible neuromodulation approach in freely moving mice. AhSonogenetics utilizes wearable ultrasound devices manufactured using 3D-printed Airy-beam holographic metasurfaces. These devices are designed to manipulate neurons genetically engineered to express ultrasound-sensitive ion channels, enabling precise modulation of specific neuronal populations. By dynamically steering the focus of Airy beams through ultrasound frequency tuning, AhSonogenetics is capable of modulating neuronal populations within specific subregions of the striatum. One notable feature of AhSonogenetics is its ability to flexibly stimulate either the left or right striatum in a single mouse. This flexibility is achieved by simply switching the acoustic metasurface in the wearable ultrasound device, eliminating the need for multiple implants or interventions. AhSonogentocs also integrates seamlessly with in vivo calcium recording via fiber photometry, showcasing its compatibility with optical modalities without cross talk. Moreover, AhSonogenetics can generate double foci for bilateral stimulation and alleviate motor deficits in Parkinson’s disease mice. This advancement is significant since many neurological disorders, including Parkinson’s disease, involve dysfunction in multiple brain regions. By enabling precise and flexible cell type–specific neuromodulation without invasive procedures, AhSonogenetics provides a powerful tool for investigating intact neural circuits and offers promising interventions for neurological disorders.
AB - Advancing our understanding of brain function and developing treatments for neurological diseases hinge on the ability to modulate neuronal groups in specific brain areas without invasive techniques. Here, we introduce Airy-beam holographic sonogenetics (AhSonogenetics) as an implant-free, cell type–specific, spatially precise, and flexible neuromodulation approach in freely moving mice. AhSonogenetics utilizes wearable ultrasound devices manufactured using 3D-printed Airy-beam holographic metasurfaces. These devices are designed to manipulate neurons genetically engineered to express ultrasound-sensitive ion channels, enabling precise modulation of specific neuronal populations. By dynamically steering the focus of Airy beams through ultrasound frequency tuning, AhSonogenetics is capable of modulating neuronal populations within specific subregions of the striatum. One notable feature of AhSonogenetics is its ability to flexibly stimulate either the left or right striatum in a single mouse. This flexibility is achieved by simply switching the acoustic metasurface in the wearable ultrasound device, eliminating the need for multiple implants or interventions. AhSonogentocs also integrates seamlessly with in vivo calcium recording via fiber photometry, showcasing its compatibility with optical modalities without cross talk. Moreover, AhSonogenetics can generate double foci for bilateral stimulation and alleviate motor deficits in Parkinson’s disease mice. This advancement is significant since many neurological disorders, including Parkinson’s disease, involve dysfunction in multiple brain regions. By enabling precise and flexible cell type–specific neuromodulation without invasive procedures, AhSonogenetics provides a powerful tool for investigating intact neural circuits and offers promising interventions for neurological disorders.
KW - Airy beam
KW - focused ultrasound
KW - hologram
KW - neuromodulation
KW - sonogenetics
UR - http://www.scopus.com/inward/record.url?scp=85196608705&partnerID=8YFLogxK
U2 - 10.1073/pnas.2402200121
DO - 10.1073/pnas.2402200121
M3 - Article
C2 - 38885384
AN - SCOPUS:85196608705
SN - 0027-8424
VL - 121
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 26
M1 - e2402200121
ER -