Aging diminishes lamellar and woven bone formation induced by tibial compression in adult C57BL/6

Nilsson Holguin, Michael D. Brodt, Michelle E. Sanchez, Matthew J. Silva

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

Aging purportedly diminishes the ability of the skeleton to respond to mechanical loading, but recent data show that old age did not impair loading-induced accrual of bone in BALB/c mice. Here, we hypothesized that aging limits the response of the tibia to axial compression over a range of adult ages in the commonly used C57BL/6. We subjected the right tibia of old (22month), middle-aged (12month) and young-adult (5month) female C57BL/6 mice to peak periosteal strains (measured near the mid-diaphysis) of -2200με and -3000με (n=12-15/age/strain) via axial tibial compression (4Hz, 1200cycles/day, 5days/week, 2weeks). The left tibia served as a non-loaded, contralateral control. In mice of every age, tibial compression that engendered a peak strain of -2200με did not alter cortical bone volume but loading to a peak strain of -3000με increased cortical bone volume due in part to woven bone formation. Both loading magnitudes increased total volume, medullary volume and periosteal bone formation parameters (MS/BS, BFR/BS) near the cortical midshaft. Compared to the increase in total volume and bone formation parameters of 5-month mice, increases were less in 12- and 22-month mice by 45-63%. Moreover, woven bone incidence was greatest in 5-month mice. Similarly, tibial loading at -3000με increased trabecular BV/TV of 5-month mice by 18% (from 0.085mm3/mm3), but trabecular BV/TV did not change in 12- or 22-month mice, perhaps due to low initial BV/TV (0.032 and 0.038mm3/mm3, respectively). In conclusion, these data show that while young-adult C57BL/6 mice had greater periosteal bone formation following loading than middle-aged or old mice, aging did not eliminate the ability of the tibia to accrue cortical bone.

Original languageEnglish
Pages (from-to)83-91
Number of pages9
JournalBone
Volume65
DOIs
StatePublished - Aug 2014

Keywords

  • Aging
  • Bone formation
  • MicroCT
  • Mouse
  • Tibial compression

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