TY - JOUR
T1 - Advances in ligand-specific biosensing for structurally similar molecules
AU - Xi, Chenggang
AU - Diao, Jinjin
AU - Moon, Tae Seok
N1 - Publisher Copyright:
© 2023 Elsevier Inc.
PY - 2023/12/20
Y1 - 2023/12/20
N2 - The specificity of biological systems makes it possible to develop biosensors targeting specific metabolites, toxins, and pollutants in complex medical or environmental samples without interference from structurally similar compounds. For the last two decades, great efforts have been devoted to creating proteins or nucleic acids with novel properties through synthetic biology strategies. Beyond augmenting biocatalytic activity, expanding target substrate scopes, and enhancing enzymes' enantioselectivity and stability, an increasing research area is the enhancement of molecular specificity for genetically encoded biosensors. Here, we summarize recent advances in the development of highly specific biosensor systems and their essential applications. First, we describe the rational design principles required to create libraries containing potential mutants with less promiscuity or better specificity. Next, we review the emerging high-throughput screening techniques to engineer biosensing specificity for the desired target. Finally, we examine the computer-aided evaluation and prediction methods to facilitate the construction of ligand-specific biosensors.
AB - The specificity of biological systems makes it possible to develop biosensors targeting specific metabolites, toxins, and pollutants in complex medical or environmental samples without interference from structurally similar compounds. For the last two decades, great efforts have been devoted to creating proteins or nucleic acids with novel properties through synthetic biology strategies. Beyond augmenting biocatalytic activity, expanding target substrate scopes, and enhancing enzymes' enantioselectivity and stability, an increasing research area is the enhancement of molecular specificity for genetically encoded biosensors. Here, we summarize recent advances in the development of highly specific biosensor systems and their essential applications. First, we describe the rational design principles required to create libraries containing potential mutants with less promiscuity or better specificity. Next, we review the emerging high-throughput screening techniques to engineer biosensing specificity for the desired target. Finally, we examine the computer-aided evaluation and prediction methods to facilitate the construction of ligand-specific biosensors.
KW - computational biomolecule design
KW - directed evolution
KW - genetically encoded biosensor
KW - library screening
KW - ligand-receptor interaction
KW - machine learning
KW - molecular specificity
KW - protein engineering
UR - http://www.scopus.com/inward/record.url?scp=85180086305&partnerID=8YFLogxK
U2 - 10.1016/j.cels.2023.10.009
DO - 10.1016/j.cels.2023.10.009
M3 - Review article
C2 - 38128482
AN - SCOPUS:85180086305
SN - 2405-4712
VL - 14
SP - 1024
EP - 1043
JO - Cell Systems
JF - Cell Systems
IS - 12
ER -