Advances in engineering genetic circuits for microbial biocontainment

Yuefeng Ma; Abhijit Manna; Tae Seok Moon

doi:10.1016/j.coisb.2023.100483

Advances in engineering genetic circuits for microbial biocontainment

Yuefeng Ma, Abhijit Manna, Tae Seok Moon

Research output: Contribution to journal › Review article › peer-review

7 Scopus citations

Abstract

The development of synthetic biology has resulted in the use of genetically engineered microbes (GEMs), becoming increasingly critical for addressing global issues such as health, food shortage, climate crisis, and environmental pollution. However, GEMs also pose a potential threat to the ecosystem, necessitating the implementation of biocontainment strategies. Synthetic genetic circuits have the potential to provide an additional level of safety and control beyond traditional physical containment measures. The development of biocontainment strategies is ongoing, including the use of kill switches, auxotrophy, and stringent response circuits, to control the viability of GEMs. This review discusses the application and future directions of genetic circuits for microbial biocontainment strategies.

Original language	English
Article number	100483
Journal	Current Opinion in Systems Biology
Volume	36
DOIs	https://doi.org/10.1016/j.coisb.2023.100483
State	Published - Dec 2023

Keywords

Genetic circuits
Kill switch
Microbial biocontainment
Microbial consortia
Stringent control

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10.1016/j.coisb.2023.100483

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title = "Advances in engineering genetic circuits for microbial biocontainment",

abstract = "The development of synthetic biology has resulted in the use of genetically engineered microbes (GEMs), becoming increasingly critical for addressing global issues such as health, food shortage, climate crisis, and environmental pollution. However, GEMs also pose a potential threat to the ecosystem, necessitating the implementation of biocontainment strategies. Synthetic genetic circuits have the potential to provide an additional level of safety and control beyond traditional physical containment measures. The development of biocontainment strategies is ongoing, including the use of kill switches, auxotrophy, and stringent response circuits, to control the viability of GEMs. This review discusses the application and future directions of genetic circuits for microbial biocontainment strategies.",

keywords = "Genetic circuits, Kill switch, Microbial biocontainment, Microbial consortia, Stringent control",

author = "Yuefeng Ma and Abhijit Manna and Moon, {Tae Seok}",

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year = "2023",

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language = "English",

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T1 - Advances in engineering genetic circuits for microbial biocontainment

AU - Ma, Yuefeng

AU - Manna, Abhijit

AU - Moon, Tae Seok

PY - 2023/12

Y1 - 2023/12

N2 - The development of synthetic biology has resulted in the use of genetically engineered microbes (GEMs), becoming increasingly critical for addressing global issues such as health, food shortage, climate crisis, and environmental pollution. However, GEMs also pose a potential threat to the ecosystem, necessitating the implementation of biocontainment strategies. Synthetic genetic circuits have the potential to provide an additional level of safety and control beyond traditional physical containment measures. The development of biocontainment strategies is ongoing, including the use of kill switches, auxotrophy, and stringent response circuits, to control the viability of GEMs. This review discusses the application and future directions of genetic circuits for microbial biocontainment strategies.

AB - The development of synthetic biology has resulted in the use of genetically engineered microbes (GEMs), becoming increasingly critical for addressing global issues such as health, food shortage, climate crisis, and environmental pollution. However, GEMs also pose a potential threat to the ecosystem, necessitating the implementation of biocontainment strategies. Synthetic genetic circuits have the potential to provide an additional level of safety and control beyond traditional physical containment measures. The development of biocontainment strategies is ongoing, including the use of kill switches, auxotrophy, and stringent response circuits, to control the viability of GEMs. This review discusses the application and future directions of genetic circuits for microbial biocontainment strategies.

KW - Genetic circuits

KW - Kill switch

KW - Microbial biocontainment

KW - Microbial consortia

KW - Stringent control

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DO - 10.1016/j.coisb.2023.100483

M3 - Review article

AN - SCOPUS:85177616615

SN - 2452-3100

VL - 36

JO - Current Opinion in Systems Biology

JF - Current Opinion in Systems Biology

M1 - 100483

ER -

Advances in engineering genetic circuits for microbial biocontainment

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