TY - JOUR
T1 - Adipose Tissue CTGF Expression is Associated with Adiposity and Insulin Resistance in Humans
AU - Yoshino, Jun
AU - Patterson, Bruce W.
AU - Klein, Samuel
N1 - Funding Information:
Funding agencies: This study was supported by National Institutes of Health grants DK 56341 (Nutrition Obesity Research Center), DK20579 (Diabetes Research Center), DK052574 (Digestive Disease Research Center), RR024992 (Clinical and Translational Science Award), and TR000450 (KL2 Career Developmental Award), as well as grants from the Pershing Square Foundation, the Barnes Jewish Hospital Foundation, and the Longer Life Foundation. Disclosure: The authors declare no conflict of interest. Clinical trial registration: ClinicalTrials.gov identifiers: NCT01184170; NCT01299519; NCT00981500; NCT01104220. Additional Supporting Information may be found in the online version of this article. Received: 21 November 2018; Accepted: 18 February 2019; Published online 19 April 2019. doi:10.1002/oby.22463
Publisher Copyright:
© 2019 The Obesity Society
PY - 2019/6
Y1 - 2019/6
N2 - Objective: Connective tissue growth factor (CTGF) is an important regulator of fibrogenesis in many organs. This study evaluated the interrelationship among adipose tissue CTGF expression, fat mass, and insulin resistance in humans. Methods: This study examined (1) CTGF gene expression in human subcutaneous preadipocytes before and after inducing adipogenesis; (2) relationships among abdominal subcutaneous adipose tissue CTGF gene expression, body fat mass, and indices of insulin sensitivity, including the hepatic insulin sensitivity index and the hyperinsulinemic-euglycemic clamp procedure in conjunction with stable isotope glucose tracer infusion, in 72 people who had marked differences in adiposity and insulin sensitivity; (3) localization of CTGF protein in subcutaneous adipose tissue; and (4) effect of progressive (5%, 11%, and 16%) weight loss on adipose tissue CTGF gene expression. Results: CTGF was highly expressed in preadipocytes, not adipocytes. Adipose tissue CTGF expression was strongly correlated with body fat mass and both skeletal muscle and liver insulin sensitivity, and CTGF-positive cells were predominantly found in areas of fibrosis. Progressive weight loss caused a stepwise decrease in adipose tissue CTGF expression. Conclusions: It was concluded that increased CTGF expression is associated with adipose tissue expansion, adipose tissue fibrosis, and multi-organ insulin resistance in people with obesity.
AB - Objective: Connective tissue growth factor (CTGF) is an important regulator of fibrogenesis in many organs. This study evaluated the interrelationship among adipose tissue CTGF expression, fat mass, and insulin resistance in humans. Methods: This study examined (1) CTGF gene expression in human subcutaneous preadipocytes before and after inducing adipogenesis; (2) relationships among abdominal subcutaneous adipose tissue CTGF gene expression, body fat mass, and indices of insulin sensitivity, including the hepatic insulin sensitivity index and the hyperinsulinemic-euglycemic clamp procedure in conjunction with stable isotope glucose tracer infusion, in 72 people who had marked differences in adiposity and insulin sensitivity; (3) localization of CTGF protein in subcutaneous adipose tissue; and (4) effect of progressive (5%, 11%, and 16%) weight loss on adipose tissue CTGF gene expression. Results: CTGF was highly expressed in preadipocytes, not adipocytes. Adipose tissue CTGF expression was strongly correlated with body fat mass and both skeletal muscle and liver insulin sensitivity, and CTGF-positive cells were predominantly found in areas of fibrosis. Progressive weight loss caused a stepwise decrease in adipose tissue CTGF expression. Conclusions: It was concluded that increased CTGF expression is associated with adipose tissue expansion, adipose tissue fibrosis, and multi-organ insulin resistance in people with obesity.
UR - http://www.scopus.com/inward/record.url?scp=85064658150&partnerID=8YFLogxK
U2 - 10.1002/oby.22463
DO - 10.1002/oby.22463
M3 - Article
C2 - 31004409
AN - SCOPUS:85064658150
VL - 27
SP - 957
EP - 962
JO - Obesity
JF - Obesity
SN - 1930-7381
IS - 6
ER -