Adipose tissue as a stem cell source for musculoskeletal regeneration

Jeffrey M. Gimble, Warren Grayson, Farshid Guilak, Mandi J. Lopez, Gordana Vunjak-Novakovic-

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Adipose tissue is an abundant, easily accessible, and reproducible cell source for musculo-skeletal regenerative medicine applications. Initial derivation steps yield a heterogeneous population of cells of stromal vascular fraction (SVF) cells. Subsequent adherent selection of the SVF results in a relatively homogeneous population of adipose-derived stromal/stem cells (ASCs) capable of adipogenic, chondrogenic, myogenic, and osteogenic differentiation in vitro on scaffolds in bioreactors and in vivo in pre-clinical animal models. Unlike hematopoietic cells, ASCs do not elicit a robust lymphocyte reaction and instead release immunosuppressive factors, such as prostaglandin E2. These immunomodulatory features suggest that allogeneic and autologous ASCs will engraft successfully for tissue regeneration purposes. The differentiation and expansion potential of ASCs can be modified by growth factors, bioinductive scaffolds, and bioreactors providing environmental control and biophysical stimulation. Gene therapy approaches using lentiviral transduction can be used to direct differentiation of ASCs to particular lineages. We discuss the utility of ASCs for musculo-skeletal tissue repair and some of the technologies that can be implemented to unlock the full regenerative potential of these highly valuable cells.

Original languageEnglish
Pages (from-to)69-81
Number of pages13
JournalFrontiers in Bioscience - Scholar
Volume3 S
Issue number1
DOIs
StatePublished - Jan 1 2011

Keywords

  • ASCs
  • Adipose stem cells
  • BMSCs
  • Bone marrowderived mesenchymal stem cells
  • Chondrocyte
  • Immunophenotype
  • Myocyte
  • Osteoblast
  • Review
  • SVF cells
  • Stromal vascular fraction
  • Tenocyte

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