TY - JOUR
T1 - Adhesive mechanisms governing interferon-producing cell recruitment into lymph nodes
AU - Diacovo, Thomas G.
AU - Blasius, Amanda L.
AU - Mak, Tak W.
AU - Cella, Marina
AU - Colonna, Marco
PY - 2005/9/5
Y1 - 2005/9/5
N2 - Natural interferon-producing cells (IPCs) are found in peripheral lymph nodes (PLNs), where they support NK cell, T cell, and B cell responses to pathogens. However, their route of entry and the adhesive mechanisms used to gain access to PLNs remain poorly defined. We report that IPCs can enter PLNs via a hematogenous route, which involves a multistep adhesive process, and that transmigration is enhanced by inflammation. Results indicate that L-selectin on IPCs is required for efficient attachment and rolling on high endothelial venules in vivo in both nonstimulated and inflamed PLNs. IPCs, however, also possess functional ligands for E-selectin that contribute to this process only in the latter case. In conjunction with selectin-mediated adhesion, both β1- and β2-integrins participate in IPC attachment to the inflamed vessel wall, whereas chemotaxis relies in part on the chemokine receptor CCR5. Identification of the adhesive machinery required for IPC trafficking into PLNs may provide opportunities to regulate immune responses reliant on the activity of these cells. JEM
AB - Natural interferon-producing cells (IPCs) are found in peripheral lymph nodes (PLNs), where they support NK cell, T cell, and B cell responses to pathogens. However, their route of entry and the adhesive mechanisms used to gain access to PLNs remain poorly defined. We report that IPCs can enter PLNs via a hematogenous route, which involves a multistep adhesive process, and that transmigration is enhanced by inflammation. Results indicate that L-selectin on IPCs is required for efficient attachment and rolling on high endothelial venules in vivo in both nonstimulated and inflamed PLNs. IPCs, however, also possess functional ligands for E-selectin that contribute to this process only in the latter case. In conjunction with selectin-mediated adhesion, both β1- and β2-integrins participate in IPC attachment to the inflamed vessel wall, whereas chemotaxis relies in part on the chemokine receptor CCR5. Identification of the adhesive machinery required for IPC trafficking into PLNs may provide opportunities to regulate immune responses reliant on the activity of these cells. JEM
UR - http://www.scopus.com/inward/record.url?scp=24344450308&partnerID=8YFLogxK
U2 - 10.1084/jem.20051035
DO - 10.1084/jem.20051035
M3 - Article
C2 - 16147979
AN - SCOPUS:24344450308
SN - 0022-1007
VL - 202
SP - 687
EP - 696
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 5
ER -