Adenylate cyclase of human parathyroid gland

Hector J. Rodriguez, Aubrey Morrison, Eduardo Slatopolsky, Saulo Klahr

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Experiments were performed on a particulate fraction from human parathyroid glands. A high activity of adenylate cyclase was detected which was linear with time and protein concentration. The enzyme had an optimum pH in the range of 7-8 and a Km for ATP of 0.44 × 10–3 M. Ca++ had a profound inhibitory effect; a concentration of 0.5 mM Ca++ reduced enzyme activity by 60%. Maximal enzyme activity was obtained with 5 mM Mg++; higher concentrations of this cation also inhibited enzyme activity. The effect of Mn++ was similar to that of Mg++. Enzyme activity was stimulated by NaF, catecholamines, glucagon, and calcitonin. The effect of catecholamines seems to be mediated through β-adrenergic receptors.

Original languageEnglish
Pages (from-to)319-325
Number of pages7
JournalJournal of Clinical Endocrinology and Metabolism
Volume47
Issue number2
DOIs
StatePublished - Aug 1978

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