TY - JOUR
T1 - Adenylate cyclase of human parathyroid gland
AU - Rodriguez, Hector J.
AU - Morrison, Aubrey
AU - Slatopolsky, Eduardo
AU - Klahr, Saulo
PY - 1978/8
Y1 - 1978/8
N2 - Experiments were performed on a particulate fraction from human parathyroid glands. A high activity of adenylate cyclase was detected which was linear with time and protein concentration. The enzyme had an optimum pH in the range of 7-8 and a Km for ATP of 0.44 × 10–3 M. Ca++ had a profound inhibitory effect; a concentration of 0.5 mM Ca++ reduced enzyme activity by 60%. Maximal enzyme activity was obtained with 5 mM Mg++; higher concentrations of this cation also inhibited enzyme activity. The effect of Mn++ was similar to that of Mg++. Enzyme activity was stimulated by NaF, catecholamines, glucagon, and calcitonin. The effect of catecholamines seems to be mediated through β-adrenergic receptors.
AB - Experiments were performed on a particulate fraction from human parathyroid glands. A high activity of adenylate cyclase was detected which was linear with time and protein concentration. The enzyme had an optimum pH in the range of 7-8 and a Km for ATP of 0.44 × 10–3 M. Ca++ had a profound inhibitory effect; a concentration of 0.5 mM Ca++ reduced enzyme activity by 60%. Maximal enzyme activity was obtained with 5 mM Mg++; higher concentrations of this cation also inhibited enzyme activity. The effect of Mn++ was similar to that of Mg++. Enzyme activity was stimulated by NaF, catecholamines, glucagon, and calcitonin. The effect of catecholamines seems to be mediated through β-adrenergic receptors.
UR - http://www.scopus.com/inward/record.url?scp=0018096348&partnerID=8YFLogxK
U2 - 10.1210/jcem-47-2-319
DO - 10.1210/jcem-47-2-319
M3 - Article
C2 - 263299
AN - SCOPUS:0018096348
SN - 0021-972X
VL - 47
SP - 319
EP - 325
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
IS - 2
ER -