Adenovirus stimulates choline efflux by increasing expression of organic cation transporter-2

Olga L. Miakotina, Marianna Agassandian, Lei Shi, Dwight C. Look, Rama K. Mallampalli

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

We examined the effect of wild-type human adenovirus (AdS) on choline transport in murine lung epithelia (MLE) and in rodent primary alveolar type II cells. Cells were active in pH-sensitive, reversible transport of choline, a process blocked pharmacologically with phenoxybenzamine, an inhibitor of organic cation transporters (OCT). PCR products for the choline transporters, OCT-1 and OCT-2, were detected, but only OCT-2 protein was robustly expressed within MLE and primary alveolar epithelial cells. Ad5 produced a two- to threefold increase in choline efflux from cells, resulting in a significant reduction in intracellular choline content and its major product, phosphatidylcholine. Effects of Ad5 on choline efflux were inhibited with phenoxybenzamine, and choline efflux was attenuated by OCT-2 small interfering RNA. Adenovirus also produced a dose-dependent increase in immunoreactive OCT-2 levels concomitant with increased cellular OCT-2 steady-state rnRNA. These results indicate that adenoviruses can significantly disrupt choline trafficking in lung epithelia by upregulating expression of an alveolar protein involved in organic cation transport.

Original languageEnglish
Pages (from-to)L93-L102
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume288
Issue number1 32-1
DOIs
StatePublished - Jan 1 2005
Externally publishedYes

Keywords

  • Organic cation transporter
  • Phenoxybenzamine

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