Adenoviral/retroviral vector chimeras: A novel strategy to achieve high- efficiency stable transduction in vivo

Guadalupe Bilbao, Meizhen Feng, Claudine Rancourt, William H. Jackson, David T. Curiel

Research output: Contribution to journalReview article

62 Scopus citations

Abstract

Gene therapy to correct defective genes requires efficient gene delivery and long-term gene expression. Realization of both goals with available vector systems has so far not been achieved. As a novel approach to solve this problem, we have developed a chimeric viral vector system that exploits favorable aspects of both adenoviral and retroviral vectors. In this schema, adenoviral vectors induce target cells to function as transient retrovital producer cells in vivo. The progeny retroviral vector particles can then effectively achieve stable transduction of neighboring cells. In this system, the nonintegrative adenoviral vector is rendered functionally integra five via the intermediate generation of an induced retroviral producer cell. Such chimeric vectors may now allow realization of the requisite goals for specific gene therapy applications.

Original languageEnglish
Pages (from-to)624-634
Number of pages11
JournalFASEB Journal
Volume11
Issue number8
StatePublished - Jul 1 1997
Externally publishedYes

Keywords

  • Adenoviral vector
  • Retrovirus
  • Transgene sequence
  • Vector chimera gene delivery

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