@article{cd578013c295493e9f1590dc4f4c27b7,
title = "Adenoviral protein V promotes a process of viral assembly through nucleophosmin 1",
abstract = "Adenoviral infection induces nucleoplasmic redistribution of a nucleolar nucleophosmin 1/NPM1/B23.1. NPM1 is preferentially localized in the nucleoli of normal cells, whereas it is also present at the nuclear matrix in cancer cells. However, the biological roles of NPM1 during infection are unknown. Here, by analyzing a pV-deletion mutant, Ad5-dV/TSB, we demonstrate that pV promotes the NPM1 translocation from the nucleoli to the nucleoplasm in normal cells, and the NPM1 translocation is correlated with adenoviral replication. Lack of pV causes a dramatic reduction of adenoviral replication in normal cells, but not cancer cells, and Ad5-dV/TSB was defective in viral assembly in normal cells. NPM1 knockdown inhibits adenoviral replication, suggesting an involvement of NPM1 in adenoviral biology. Further, we show that NPM1 interacts with empty adenovirus particles which are an intermediate during virion maturation by immunoelectron microscopy. Collectively, these data implicate that pV participates in a process of viral assembly through NPM1.",
keywords = "Adenoviral assembly, Adenovirus, Cancer gene therapy, Nucleophosmin 1, Protein V",
author = "Hideyo Ugai and Dobbins, {George C.} and Minghui Wang and Le, {Long P.} and Matthews, {David A.} and Curiel, {David T.}",
note = "Funding Information: We thank Dr. Roger Y. Tsien (University of California at San Diego) for providing the mRFP1 construct, and Drs A. J. Levine (The Cancer Institute of New Jersey), W. S. M. Wold (St. Louis University), T. Shenk (Princeton University) for providing antibodies against adenoviral proteins. We are also grateful to Drs. Melissa F. Chimento and Olga Borkhsenious for technical support with transmission electron microscopy at the High Resolution Imaging Facility in University of Alabama at Birmingham and School of Veterinary Medicine Microscopy Center in Louisiana State University, respectively. This work was supported by grants from Susan G. Komen for the Cure PDF0707736 (Dr. Hideyo Ugai), Susan G. Komen for the Cure KG100194 (Drs. David T. Curiel and Hideyo Ugai), Grant number 083604 from the Wellcome Trust (Dr. David A. Matthews), grant T32-NS048039 from the National Institutes of Health (Dr. George C. Dobbins), and grant R01CA121187 from the National Institutes of Health (Dr. David T. Curiel). Appendix A ",
year = "2012",
month = oct,
day = "25",
doi = "10.1016/j.virol.2012.05.028",
language = "English",
volume = "432",
pages = "283--295",
journal = "Virology",
issn = "0042-6822",
number = "2",
}