Immunohistochemistry was performed on lung tissue obtained from patients with severe unexplained pulmonary hypertension using an antibody to the amino-terminal end of the procollagen type I propeptide. This antibody identifies newly synthesized αI(I) procollagen before cleavage of the amino- terminal propeptide following secretion and, therefore, can identify sites of active collagen deposition. Procollagen was detected in the media, media and neointima, or neointima alone of a large number of small muscular arteries from hypertensive lungs. Normal adult lungs were negative. Neointimal cells in remodeled small muscular arteries stained positively for α-smooth muscle actin and desmin consistent with a smooth muscle lineage. These data suggest smooth musclelike cells in small muscular arteries are actively synthesizing collagen in patients with severe unexplained pulmonary hypertension.

Original languageEnglish
Pages (from-to)121-129
Number of pages9
JournalAmerican Journal of Pathology
Issue number1
StatePublished - 1993


Dive into the research topics of 'Active collagen synthesis by pulmonary arteries in human primary pulmonary hypertension'. Together they form a unique fingerprint.

Cite this