Activation of Raf-1 by 14-3-3 proteins

Wendy J. Fantl; Anthony J. Muslin; Akira Kikuchi; Jay A. Martin; Angus M. MacNicol; Richard W. Grosst; Lewis T. Williams

doi:10.1038/371612a0

Activation of Raf-1 by 14-3-3 proteins

Wendy J. Fantl, Anthony J. Muslin, Akira Kikuchi, Jay A. Martin, Angus M. MacNicol, Richard W. Grosst, Lewis T. Williams

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Abstract

THE protein Raf-1, a key mediator of mitogenesis and differentia-tion, associates with p21^ras (refs 1-3). However, the regulation of the serine/threonine kinase activity of Raf-1 is still not understood4 13. Using the yeast two-hybrid system8'14-16, we identi-fied two structurally related proteins that interact with the amino-terminal region of Raf-1. These proteins, 14-3-3 £ (PLA2) and 14-3-3 p (HS1), are members of the 14-3-3 family of proteins17'23. Expression of 14-3-3 proteins in Xenopm oocytes enhanced Raf-1 activity and promoted Raf-1-dependent oocyte maturation. A dominant negative mutant of Raf-1 blocked the effects of 14-3-3 protein.

Original language	English
Pages (from-to)	612-614
Number of pages	3
Journal	Nature
Volume	371
Issue number	6498
DOIs	https://doi.org/10.1038/371612a0
State	Published - 1994

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title = "Activation of Raf-1 by 14-3-3 proteins",

abstract = "THE protein Raf-1, a key mediator of mitogenesis and differentia-tion, associates with p21ras (refs 1-3). However, the regulation of the serine/threonine kinase activity of Raf-1 is still not understood4 13. Using the yeast two-hybrid system8'14-16, we identi-fied two structurally related proteins that interact with the amino-terminal region of Raf-1. These proteins, 14-3-3 £ (PLA2) and 14-3-3 p (HS1), are members of the 14-3-3 family of proteins17'23. Expression of 14-3-3 proteins in Xenopm oocytes enhanced Raf-1 activity and promoted Raf-1-dependent oocyte maturation. A dominant negative mutant of Raf-1 blocked the effects of 14-3-3 protein.",

author = "Fantl, {Wendy J.} and Muslin, {Anthony J.} and Akira Kikuchi and Martin, {Jay A.} and MacNicol, {Angus M.} and Grosst, {Richard W.} and Williams, {Lewis T.}",

year = "1994",

doi = "10.1038/371612a0",

language = "English",

volume = "371",

pages = "612--614",

journal = "Nature",

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T1 - Activation of Raf-1 by 14-3-3 proteins

AU - Fantl, Wendy J.

AU - Muslin, Anthony J.

AU - Kikuchi, Akira

AU - Martin, Jay A.

AU - MacNicol, Angus M.

AU - Grosst, Richard W.

AU - Williams, Lewis T.

PY - 1994

Y1 - 1994

N2 - THE protein Raf-1, a key mediator of mitogenesis and differentia-tion, associates with p21ras (refs 1-3). However, the regulation of the serine/threonine kinase activity of Raf-1 is still not understood4 13. Using the yeast two-hybrid system8'14-16, we identi-fied two structurally related proteins that interact with the amino-terminal region of Raf-1. These proteins, 14-3-3 £ (PLA2) and 14-3-3 p (HS1), are members of the 14-3-3 family of proteins17'23. Expression of 14-3-3 proteins in Xenopm oocytes enhanced Raf-1 activity and promoted Raf-1-dependent oocyte maturation. A dominant negative mutant of Raf-1 blocked the effects of 14-3-3 protein.

AB - THE protein Raf-1, a key mediator of mitogenesis and differentia-tion, associates with p21ras (refs 1-3). However, the regulation of the serine/threonine kinase activity of Raf-1 is still not understood4 13. Using the yeast two-hybrid system8'14-16, we identi-fied two structurally related proteins that interact with the amino-terminal region of Raf-1. These proteins, 14-3-3 £ (PLA2) and 14-3-3 p (HS1), are members of the 14-3-3 family of proteins17'23. Expression of 14-3-3 proteins in Xenopm oocytes enhanced Raf-1 activity and promoted Raf-1-dependent oocyte maturation. A dominant negative mutant of Raf-1 blocked the effects of 14-3-3 protein.

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Activation of Raf-1 by 14-3-3 proteins

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