Activation of natural killer cells by the mAb YTA-1 that recognizes leukocyte function-associated antigen-1

Katsuji Sugle, Kazuhiro Nakamura, Keisuke Teshigawara, Michael S. Diamond, Timothy A. Springer, Yoshiaki Nakamura, Warren J. Leonard, Atsushi Uchida, Junji Yodoi

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7 Scopus citations


The mAb YTA-1, which brightly stains CD3-CD16+ large granular lymphocytes (LGL)/natural killer (NK) cells and CD8+ T cells by immunofluorescence, is specific for leukocyte function-associated antigen (LFA)-1. Some mAbs recognizing the LFA-1α chain (CD11a) or LFA-1β chain (CD18) inhibited the binding of YTA-1 to peripheral blood mononuclear cells. YTA-1 mAb could be chemically cross-linked to 170 and 96 kDa molecules, whose molecular weights correspond to those of LFA-1α and β respectively.YTA-1 bound to COS-7 cells co-transfected with CD11a and CD18 cDNAs, but not to untransfected cells. Reactivities of YTA-1 to K562 cells transfected with LFA-1α and β(CD11a/CD18) cDNAs and to CHO cells transfected with Mac-1 (CD11b/CD18) or p150, 95 (CD11c/CD18) cDNAs strongly suggest that YTA-1 recognizes either LFA-1α or an epitope formed by a combination of LFA-1α and β. Treatment of fresh CD3-CD16+ LGL with YTA-1 augmented cytolytic activity and induced proliferation. F(ab')2 fragments of YTA-1 augmented NK cytotoxicity, indicating that the NK activating signal was transmitted through LFA-1 without involvement of Fcγ receptor III. In contrast, the other mAbs against LFA-1 could not activate NK cells. These results collectively indicate that YTA-1 recognizes a unique epitope of LFA-1, which is involved in activation of fresh NK cells.

Original languageEnglish
Pages (from-to)763-769
Number of pages7
JournalInternational Immunology
Issue number5
StatePublished - May 1995


  • LFA-1
  • Natural killer
  • YT cell line


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