Activated platelets express IL-1 activity

C. M. Hawrylowicz, S. A. Santoro, F. M. Platt, E. R. Unanue

Research output: Contribution to journalArticlepeer-review

114 Scopus citations

Abstract

Suspensions of washed human platelets express IL-1 activity after activation with agents such as thrombin, collagen, ADP, or epinephrine as judged by the ability of the platelet suspensions to support the growth of a T cell line, D10.G4.1, which exhibits a growth requirement for IL-1. Unactivated platelets express little IL-1 activity. The IL-1 activity expressed by activated platelets appears to be entirely associated with the platelet surface. No IL-1 activity was detected in supernatants derived from suspensions of activated platelets. A mAb specific for IL-1β inhibited 90% of the activity expressed by thrombin-activated platelets, whereas a mAb specific for IL-1α inhibited approximately 20% of the activity. A control mAb was without an effect. These results indicate that activated platelets express surface-associated IL-1 activity. Platelet surface IL-1 may provide a mechanism for altering in an extremely localized and rapid manner the properties of IL-1 responsive cells with which platelets come in direct contact during processes of inflammation and vessel wall damage.

Original languageEnglish
Pages (from-to)4015-4018
Number of pages4
JournalJournal of Immunology
Volume143
Issue number12
StatePublished - 1989

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