Activated c-Ha-ras oncogene with a guanine to thymine transversion at the twelfth codon in a human stomach cancer cell line

Guoren Deng; Shiming Chen; Jing Miao; Hua Li; Hong Cai; Peinan Liu

Activated c-Ha-ras oncogene with a guanine to thymine transversion at the twelfth codon in a human stomach cancer cell line

Guoren Deng
, Shiming Chen
, Jing Miao
, Hua Li
, Hong Cai
, Peinan Liu

Research output: Contribution to journal › Article › peer-review

Abstract

The rat fibroblast cell line Rat 1 was transfected with total DNA of a gastrocarcinoma cell line, BGC-823. The transforming gene was cloned from the genomic library of the secondary transformants using in situ hybridization with a probe of the human Alu repeat sequence. This cloned gene is homologous to the protooncogene c-Ha-ras. The activation lesion of the transforming gene was identified by sequence analysis as a single nucleotide substitution of thymine for guanine in the 12th codon. This results in the substitution of valine for glycine at the 12th amino acid of the M_r21,000 protein.

Original language	English
Pages (from-to)	3195-3198
Number of pages	4
Journal	Cancer research
Volume	47
Issue number	12
State	Published - Jun 15 1987

Cite this

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title = "Activated c-Ha-ras oncogene with a guanine to thymine transversion at the twelfth codon in a human stomach cancer cell line",

abstract = "The rat fibroblast cell line Rat 1 was transfected with total DNA of a gastrocarcinoma cell line, BGC-823. The transforming gene was cloned from the genomic library of the secondary transformants using in situ hybridization with a probe of the human Alu repeat sequence. This cloned gene is homologous to the protooncogene c-Ha-ras. The activation lesion of the transforming gene was identified by sequence analysis as a single nucleotide substitution of thymine for guanine in the 12th codon. This results in the substitution of valine for glycine at the 12th amino acid of the Mr21,000 protein.",

author = "Guoren Deng and Shiming Chen and Jing Miao and Hua Li and Hong Cai and Peinan Liu",

year = "1987",

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T1 - Activated c-Ha-ras oncogene with a guanine to thymine transversion at the twelfth codon in a human stomach cancer cell line

AU - Deng, Guoren

AU - Chen, Shiming

AU - Miao, Jing

AU - Li, Hua

AU - Cai, Hong

AU - Liu, Peinan

PY - 1987/6/15

Y1 - 1987/6/15

N2 - The rat fibroblast cell line Rat 1 was transfected with total DNA of a gastrocarcinoma cell line, BGC-823. The transforming gene was cloned from the genomic library of the secondary transformants using in situ hybridization with a probe of the human Alu repeat sequence. This cloned gene is homologous to the protooncogene c-Ha-ras. The activation lesion of the transforming gene was identified by sequence analysis as a single nucleotide substitution of thymine for guanine in the 12th codon. This results in the substitution of valine for glycine at the 12th amino acid of the Mr21,000 protein.

AB - The rat fibroblast cell line Rat 1 was transfected with total DNA of a gastrocarcinoma cell line, BGC-823. The transforming gene was cloned from the genomic library of the secondary transformants using in situ hybridization with a probe of the human Alu repeat sequence. This cloned gene is homologous to the protooncogene c-Ha-ras. The activation lesion of the transforming gene was identified by sequence analysis as a single nucleotide substitution of thymine for guanine in the 12th codon. This results in the substitution of valine for glycine at the 12th amino acid of the Mr21,000 protein.

UR - https://www.scopus.com/pages/publications/0023223152

M3 - Article

C2 - 3034404

AN - SCOPUS:0023223152

SN - 0008-5472

VL - 47

SP - 3195

EP - 3198

JO - Cancer research

JF - Cancer research

IS - 12

ER -

Activated c-Ha-ras oncogene with a guanine to thymine transversion at the twelfth codon in a human stomach cancer cell line

Abstract

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