Actions of (-)-baclofen on rat dorsal horn neurons

Ivan Kangrga, Minchun Jiang, Mirjana Randić

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101 Scopus citations


The actions of a γ-aminobutyric acid B (GABAB) agonist, (-)-baclofen, on the electrophysiological properties of neurons and synaptic transmission in the spinal dorsal horn (laminae I-IV) were examined by using intracellular recordings in spinal cord slice from young rats. In addition, the effects of baclofen on the dorsal root stimulation-evoked outflow of glutamate and aspartate from the spinal dorsal horn were examined by using high performance liquid chromatography (HPLC) with fluorimetric detection. Superfusion of baclofen (5 nM to 10 μM) hyperpolarized, in a stereoselective and bicuculline-insensitive manner, the majority (86%) of tested neurons. The hyperpolarization was associated with a decrease in membrane resistance and persisted in a nominally zero-Ca2+, 10 mM Mg2+- or a TTX-containing solution. Our findings indicate that the hyperpolarizing effect of baclofen is probably due to an increase in conductance to potassium ions. Baclofen decreased the direct excitability of dorsal horn neurons, enhanced accommadation of spike discharge, and reduced the duration of Ca2+-dependent action potentials. Baclofen depressed, or blocked, excitatory postsynaptic potentials evoked by electrical stimulation of the dorsal roots. Spontaneously occurring synaptic potentials were also reversibly deppessed by baclofen. Whereas baclofen did not produce any consistent change in the rat of the basal outflow of glutamate and aspartate, the stimulation-evoked release of the amino acids was blocked. The present results suggest that baclofen, by activating GABAB receptors, may modulate spinal afferent processing in the superficial dorsal horn by at least two mechanisms: (1) baclofen depresses excitatory synaptic transmission primarily by a presynaptic mechanism involving a decrease in the release of excitatory amino acids, and (2) at higher concentrations, the hyperpolarization and increased membrane conductance may contribute to the depressant effect of baclofen on excitatory synaptic transmission in the rat spinal dorsal horn.

Original languageEnglish
Pages (from-to)265-275
Number of pages11
JournalBrain Research
Issue number2
StatePublished - Oct 25 1991


  • Aspartate release
  • Baclofen
  • Glutamate release
  • Rat dorsal horn slice
  • Synaptic transmission
  • γ-Aminobutyric acid B receptor


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