TY - JOUR
T1 - Actin polymerization under pressure
T2 - A theoretical study
AU - Artyomov, Maxim N.
AU - Freed, Karl F.
N1 - Funding Information:
The authors are grateful to Jacek Dudowicz for helpful discussions and for comments on the manuscript. They would like to thank to Professor Sandra Greer for providing experimental data prior to publication. This research is supported, in part, by NSF Grant No. CHE-0416017.
PY - 2007
Y1 - 2007
N2 - An extended Flory-Huggins-type equilibrium polymerization theory for compressible systems is used to describe experimental data for the unusual pressure and temperature dependence of the equilibrium polymerization of G-actin to F-actin. The calculations provide rich insights into the reaction mechanism and the thermodynamics of actin polymerization at the molecular level. Volume changes associated with individual steps of the mechanism are calculated to be Δ Vactiv = (s1* - s1) 0 =+1553 mlmol for the activation reaction, Δ Vdim = (s2 - s1*) 0 =-3810 mlmol for dimerization, and Δ Vprop = (sP - s1) 0 =+361 mlmol for the propagation reaction, where s1 0, s1* 0, s2 0, and sP 0 are the monomer volumes in the G-actin monomer, the activated G-action, the dimer, and higher polymers, respectively. Comparison with experimental measurements is made, and discrepancies are discussed.
AB - An extended Flory-Huggins-type equilibrium polymerization theory for compressible systems is used to describe experimental data for the unusual pressure and temperature dependence of the equilibrium polymerization of G-actin to F-actin. The calculations provide rich insights into the reaction mechanism and the thermodynamics of actin polymerization at the molecular level. Volume changes associated with individual steps of the mechanism are calculated to be Δ Vactiv = (s1* - s1) 0 =+1553 mlmol for the activation reaction, Δ Vdim = (s2 - s1*) 0 =-3810 mlmol for dimerization, and Δ Vprop = (sP - s1) 0 =+361 mlmol for the propagation reaction, where s1 0, s1* 0, s2 0, and sP 0 are the monomer volumes in the G-actin monomer, the activated G-action, the dimer, and higher polymers, respectively. Comparison with experimental measurements is made, and discrepancies are discussed.
UR - http://www.scopus.com/inward/record.url?scp=33846253163&partnerID=8YFLogxK
U2 - 10.1063/1.2409928
DO - 10.1063/1.2409928
M3 - Article
C2 - 17228974
AN - SCOPUS:33846253163
SN - 0021-9606
VL - 126
JO - Journal of Chemical Physics
JF - Journal of Chemical Physics
IS - 2
M1 - 024908
ER -