Acoustofluidic platform for in-channel immunoassays

Michael M. Binkley, Mingyang Cui, Jennifer Yantis, Shamus P. Keeler, Benjamin J. Gerovac, Derek E. Byers, Michael J. Holtzman, J. Mark Meacham

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We demonstrate proof of concept for a point-of-care diagnostic that is used for the detection of chloride channel accessory 1 (CLCA1), a key regulator of mucus production. The prototypical device utilizes ultrasound-confined polystyrene (PS) microspheres held in a longitudinal standing bulk acoustic wave (LSBAW) as reaction substrates. Pressure field amplification between two included pillar arrays enables immobilization of these antigen-coated beads in a predetermined low-pressure region perpendicular to the direction of flow. Bronchoalveolar lavage (BAL) samples from IL-13 stimulated pigs were incubated overnight in a solution of untreated PS beads prior to channel introduction. A PZT- 8 piezoceramic transducer was used to actuate the channel (f1,E = 575 kHz) to focus and confine the beads in a linear zero-pressure node. A solution of two proprietary anti-CLCA1 monoclonal antibodies (mAbs) modified with sulfocyanine3 (Cy3) NHS ester were flowed (7 μL/min, 15 min) into the channel and incubated for 1.5 hours. A solution of phosphate-buffered saline was then used to remove excess antibodies prior to channel/bead cluster imaging. The bead solution was collected, under no acoustic actuation, at the outlet for analysis by flow cytometry. Increased fluorescence over control samples (p<0.0001) demonstrated that the LSBAW platform can serve as a functional immunoassay to allow for serial, contactless reagent washes and fluorescent probe introduction.

Original languageEnglish
Title of host publicationReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XI
EditorsSamuel Achilefu, Ramesh Raghavachari
PublisherSPIE
ISBN (Electronic)9781510624283
DOIs
StatePublished - 2019
EventReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XI 2019 - San Francisco, United States
Duration: Feb 4 2019Feb 5 2019

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10893
ISSN (Print)1605-7422

Conference

ConferenceReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XI 2019
CountryUnited States
CitySan Francisco
Period02/4/1902/5/19

Keywords

  • Acoustics
  • Acoustofluidics
  • Biosensing
  • Diagnostics
  • Immunoassay
  • Lab-on-a-chip
  • Microfluidics

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