@inproceedings{51133f2f55e84659a778d498a09838b6,
title = "Acoustofluidic platform for in-channel immunoassays",
abstract = "We demonstrate proof of concept for a point-of-care diagnostic that is used for the detection of chloride channel accessory 1 (CLCA1), a key regulator of mucus production. The prototypical device utilizes ultrasound-confined polystyrene (PS) microspheres held in a longitudinal standing bulk acoustic wave (LSBAW) as reaction substrates. Pressure field amplification between two included pillar arrays enables immobilization of these antigen-coated beads in a predetermined low-pressure region perpendicular to the direction of flow. Bronchoalveolar lavage (BAL) samples from IL-13 stimulated pigs were incubated overnight in a solution of untreated PS beads prior to channel introduction. A PZT- 8 piezoceramic transducer was used to actuate the channel (f1,E = 575 kHz) to focus and confine the beads in a linear zero-pressure node. A solution of two proprietary anti-CLCA1 monoclonal antibodies (mAbs) modified with sulfocyanine3 (Cy3) NHS ester were flowed (7 μL/min, 15 min) into the channel and incubated for 1.5 hours. A solution of phosphate-buffered saline was then used to remove excess antibodies prior to channel/bead cluster imaging. The bead solution was collected, under no acoustic actuation, at the outlet for analysis by flow cytometry. Increased fluorescence over control samples (p<0.0001) demonstrated that the LSBAW platform can serve as a functional immunoassay to allow for serial, contactless reagent washes and fluorescent probe introduction.",
keywords = "Acoustics, Acoustofluidics, Biosensing, Diagnostics, Immunoassay, Lab-on-a-chip, Microfluidics",
author = "Binkley, {Michael M.} and Mingyang Cui and Jennifer Yantis and Keeler, {Shamus P.} and Gerovac, {Benjamin J.} and Byers, {Derek E.} and Holtzman, {Michael J.} and {Mark Meacham}, J.",
note = "Funding Information: This research was supported by Washington University School of Engineering and Applied Sciences Collaboration Initiation Grant and. We thank the technical staff at the Institute of Materials Science and Engineering at Washington University in St. Louis for help in device fabrication, as well as the researchers at the Flow Cytometry and Fluorescence Activated Cell Sorting Core at Washington University School of Medicine in St. Louis for aiding in data collection and access to facilities. Publisher Copyright: {\textcopyright} 2019 SPIE.; null ; Conference date: 04-02-2019 Through 05-02-2019",
year = "2019",
doi = "10.1117/12.2510887",
language = "English",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Samuel Achilefu and Ramesh Raghavachari",
booktitle = "Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XI",
}