TY - JOUR
T1 - Acidophil bodies in nonalcoholic steatohepatitis
AU - Nonalcoholic Steatohepatitis Clinical Research Network
AU - Yeh, Matthew M.
AU - Belt, Patricia
AU - Brunt, Elizabeth M.
AU - Kowdley, Kris V.
AU - Wilson, Laura A.
AU - Ferrell, Linda
N1 - Funding Information:
National Institute of Diabetes and Digestive and Kidney Diseases–sponsored NASH CRN and NASH CRN Pathology Committee: Cynthia A. Behling, MD, PhD, University of California at San Diego Elizabeth M. Brunt, MD, Washington University Melissa J. Contos, MD, Virginia Commonwealth University Oscar W. Cummings, MD, Indiana University Linda D. Ferrell, MD, University of California at San Francisco Marcia Gottfried, MD, Duke University Cynthia D. Guy, MD, Duke University David E. Kleiner, MD, PhD, National Cancer Institute Yao-Chang Liu, MD, Case Western Reserve University Michael S. Torbenson, MD, Johns Hopkins University Matthew M. Yeh, MD, PhD, University of Washington Members of the NASH CRN: Baylor College of Medicine, Houston, TX: Stephanie H. Abrams, MD, MS (2007-2013); Sarah Barlow, MD; Ryan Himes, MD; Rajesh Krisnamurthy, MD; Leanel Maldonado, RN (2007-2012); Rory Mahabir Case Western Reserve University Clinical Centers: • MetroHealth Medical Center, Cleveland, OH: Srinivasan Dasarathy, MD; Jaividhya Dasarathy, MD; Carol Hawkins, RN; Arthur J. McCullough, MD • Cleveland Clinic Foundation, Cleveland, OH: Srinivasan Dasarathy, MD; Arthur J. McCullough, MD; MangeshPagadala, MD; Rish Pai, MD; Ruth Sargent, LPN Cincinnati Children’s Hospital Medical Center, Cincinnati, OH: Kimberlee Bernstein, BS, CCRP; Kristin Bramlage, MD; Kim Cecil, PhD; Stephanie DeVore, MSPH (2009-2011); Rohit Kohli, MD; Kathleen Lake, MSW (2009-2012); Daniel Podberesky, MD (2009-2014);Alex Towbin, MD; Stavra Xanthakos, MD Columbia University, New York, NY: Gerald Behr, MD; Joel E. Lavine, MD, PhD; Jay H. Lefkowitch, MD; Ali Mencin, MD; Elena Reynoso, MD Duke University Medical Center, Durham, NC: Manal F. Abdelmalek, MD, MPH; Mustafa Bashir, MD; Stephanie Buie; Anna Mae Diehl, MD; Cynthia Guy, MD; Christopher Kigongo, MB, CHB; Yi-Ping Pan; Dawn Piercy, MS, FNP; Mariko Kopping, MS, RD; Tyler Thrasher Emory University, Atlanta, GA: Adina Alazraki, MD; Rebecca Cleeton, MPH, CCRP; Saul Karpen, MD, PhD; Jessica Cruz Munos (2013-2015); Nicholas Raviele (2012-2014); Miriam Vos, MD, MSPH, FAHA Indiana University School of Medicine, Indianapolis, IN: Molly Bozic, MD; Naga Chalasani, MD; Oscar W. Cummings, MD; Samer Gawrieh, MD; Marwan Ghabril, MD; Ann Klipsch, RN; Jean P. Molleston, MD; Sarah Munson, RN; Linda Ragozzino, RN; Kumar Sandrasegaran, MD; GirishSubbarao, MD; Raj Vuppalanchi, MD Johns Hopkins Hospital, Baltimore, MD: Kimberly Kafka, RN; Ann Scheimann, MD Northwestern University Feinberg School of Medicine/Ann & Robert H. Lurie Children’s Hospital of Chicago: KatieAmsden, MPH; Mark H. Fishbein, MD; Elizabeth Kirwan, RN; Saeed Mohammad, MD; Cynthia Rigsby, MD; Lisa Sharda, RD; Peter F. Whitington, MD Saint Louis University, St Louis, MO: Jose Derdoy, MD (2007-2011); Ajay Jain MD; Debra King, RN; Pat Osmack; Joan Siegner, RN (2004-2015); Susan Stewart, RN (2004-2015); Brent A. Neuschwander-Tetri, MD; Susan Torretta; Kristina Wriston, RN Swedish Medical Center, Seattle, WA: Fereshteh Assadian; Vanessa Barone; Kris V. Kowdley, MD; Alana Saddic, NP; Cara Wiseman University at Buffalo, Buffalo, NY: Susan S. Baker, MD, PhD; Sonja Williams; Lixin Zhu, PhD University of California San Diego, San Diego, CA: Jonathon Africa, MD; Brandon Ang; Jorge Angeles, MD; Sandra Arroyo, MD; Hannah Awai, MD; Cynthia Behling, MD, PhD; Archana Bhatt; Craig Bross; Janis Durelle; Rohit Loomba, MD, MHSc; Michael Middleton, MD, PhD; Kimberly Newton, MD; Melissa Paiz; Jennifer Sanford; Jeffrey B. Schwimmer, MD; Claude Sirlin, MD; Patricia Ugalde-Nicalo, MD; Mariana Dominguez Villarreal University of California San Francisco, San Francisco, CA: Bradley Aouizerat, PhD; Nathan M. Bass, MD, PhD (2002-2011); Danielle Brandman, MD, MAS; Jesse Courtier, MD; Linda D. Ferrell, MD; Shannon Fleck, MPH; Ryan Gill, MD, PhD; Bilal Hameed, MD; Camille Langlois, MS; Jacqueline Maher, MD; Emily Rothbaum Perito, MD; Claudia Ramos, MS; Philip Rosenthal, MD; Norah Terrault, MD, MPH; Patrika Tsai, MD; Ashley Ungermann, MS University of California San Francisco-Fresno, Fresno, CA: Pradeep Atla, MD; Brandon Croft; Rebekah Garcia; Sonia Garcia; Muhammad Sheikh, MD; Mandeep Singh, MD University of Washington Medical Center and Seattle Children’s Hospital, Seattle, WA: Kara Cooper; Simon Horslen, MB ChB; Evelyn Hsu, MD; Karen Murray, MD; Randolph Otto, MD; Matthew Yeh, MD, PhD; Melissa Young Virginia Commonwealth University, Richmond, VA: Sherry Boyett, RN, BSN; Laura Carucci, MD (2011-2014); Melissa J. Contos, MD; Sherri Kirwin; Kenneth Kraft, PhD (2011-2014); Velimir A. C. Luketic, MD; Puneet Puri, MD; Arun J. Sanyal, MD; Jolene Schlosser, RN, BSN; Mohhamad S. Siddiqui, MD; Ben Wolford (2011-2013) Washington University, St. Louis, MO: Elizabeth M. Brunt, MD; Kathryn Fowler, MD Resource centers: National Cancer Institute, Bethesda, MD: David E. Kleiner, MD, PhD National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD: Edward C. Doo, MD; Sherry Hall, MS; Jay H. Hoofnagle, MD; Patricia R. Robuck, PhD, MPH (2002-2011); AverellSherker, MD; Rebecca Torrance, RN, MS Johns Hopkins University, Bloomberg School of Public Health (Data Coordinating Center), Baltimore, MD: Patricia Belt, BS; Jeanne M. Clark, MD, MPH; Michele Donithan, MHS; Erin Hallinan, MHS; Milana Isaacson, BS; Kevin P. May, MS; Laura Miriel, BS; Alice Sternberg, ScM; James Tonascia, PhD; Mark Van Natta, MHS; Ivana Vaughn, MPH; Laura Wilson, ScM; Katherine Yates, ScM.
Publisher Copyright:
© 2016 Elsevier Inc. All rights reserved.
PY - 2016/6
Y1 - 2016/6
N2 - Summary The significance of the quantity of acidophil bodies (AB) in nonalcoholic steatohepatitis (NASH) is not certain. We quantified AB in liver biopsies and examined the association with the diagnosis of NASH and other histologic features. We reviewed 157 liver biopsies from the NASH Clinical Research Network Database collected in 2006. One hundred twenty-seven biopsies were from adult patients. Diagnoses were 94 definite NASH, 40 borderline NASH, and 23 definitely not NASH. The total length and average width of the core biopsies were measured, and the biopsy areas were calculated (mm2). Total AB were counted, and mean AB count per mm2 was calculated (AB/mm2) to derive acidophil body index (ABI). ABI was 0.04 (±0.08) in definite NASH and 0.02 (±0.05) in borderline/definitely not NASH groups combined (P =.02) in all 157 biopsies; similar findings were present in the 127 adult-only biopsies (0.04 ± 0.05 and 0.02 ± 0.05, respectively; P =.05). In all 157 biopsies, increased ABI was associated with greater lobular inflammation (P =.01) and many ballooned hepatocytes (P =.048). There was a positive relationship between ABI and high nonalcoholic fatty liver disease activity scores, but this association was not statistically significant. There was no association between ABI and steatosis or fibrosis stage either in the entire cohorts or in the subset of adult patients. In conclusion, the density of AB is associated with lobular inflammation, ballooned hepatocytes, and the diagnosis of NASH in adult and pediatric liver biopsies, suggesting the implication of the apoptotic pathway in NASH-associated liver cell injury.
AB - Summary The significance of the quantity of acidophil bodies (AB) in nonalcoholic steatohepatitis (NASH) is not certain. We quantified AB in liver biopsies and examined the association with the diagnosis of NASH and other histologic features. We reviewed 157 liver biopsies from the NASH Clinical Research Network Database collected in 2006. One hundred twenty-seven biopsies were from adult patients. Diagnoses were 94 definite NASH, 40 borderline NASH, and 23 definitely not NASH. The total length and average width of the core biopsies were measured, and the biopsy areas were calculated (mm2). Total AB were counted, and mean AB count per mm2 was calculated (AB/mm2) to derive acidophil body index (ABI). ABI was 0.04 (±0.08) in definite NASH and 0.02 (±0.05) in borderline/definitely not NASH groups combined (P =.02) in all 157 biopsies; similar findings were present in the 127 adult-only biopsies (0.04 ± 0.05 and 0.02 ± 0.05, respectively; P =.05). In all 157 biopsies, increased ABI was associated with greater lobular inflammation (P =.01) and many ballooned hepatocytes (P =.048). There was a positive relationship between ABI and high nonalcoholic fatty liver disease activity scores, but this association was not statistically significant. There was no association between ABI and steatosis or fibrosis stage either in the entire cohorts or in the subset of adult patients. In conclusion, the density of AB is associated with lobular inflammation, ballooned hepatocytes, and the diagnosis of NASH in adult and pediatric liver biopsies, suggesting the implication of the apoptotic pathway in NASH-associated liver cell injury.
KW - Acidophil bodies
KW - Acidophil body index
KW - Ballooning
KW - Lobular inflammation
KW - Nonalcoholic steatohepatitis
KW - Steatosis
UR - http://www.scopus.com/inward/record.url?scp=84962262958&partnerID=8YFLogxK
U2 - 10.1016/j.humpath.2016.01.001
DO - 10.1016/j.humpath.2016.01.001
M3 - Article
C2 - 26980020
AN - SCOPUS:84962262958
SN - 0046-8177
VL - 52
SP - 28
EP - 37
JO - Human Pathology
JF - Human Pathology
ER -