Accurate measurement of nicotinamide adenine dinucleotide (NAD+) with high-performance liquid chromatography

Jun Yoshino, Shin Ichiro Imai

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

39 Scopus citations

Abstract

Nicotinamide adenine dinucleotide (NAD+) plays a critical role in regulating numerous biological and physiological pathways including metabolism, inflammation, cancer, and aging in mammals. Here we describe a highly quantitative method with reverse-phase high-performance liquid chromatography (HPLC) for the determination of NAD+ levels in cells and tissues. This methodology provides accurate, reliable, and reproducible results of NAD+ measurement, which enables us to analyze various pathophysiological changes in NAD+ levels in vitro and in vivo.

Original languageEnglish
Title of host publicationSirtuins
Subtitle of host publicationMethods and Protocols
EditorsMatthew Hirschey
Pages203-215
Number of pages13
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1077
ISSN (Print)1064-3745

Keywords

  • HPLC
  • NAMPT
  • Nicotinamide adenine dinucleotide (NAD)

Fingerprint

Dive into the research topics of 'Accurate measurement of nicotinamide adenine dinucleotide (NAD<sup>+</sup>) with high-performance liquid chromatography'. Together they form a unique fingerprint.

Cite this