TY - JOUR
T1 - Accessory cell requirement in the proliferative response of T lymphocytes to hemocyanin
AU - Kammer, Gary M.
AU - Unanue, Emil R.
N1 - Funding Information:
’ This work was supported by National Institutes of Health Grants CA 14732 and AI 14723 and by a grant from The Council for Tobacco Research. 2 Present address: Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106. Reprint requests should be addressed to either author at Harvard Medical School.
PY - 1980/3
Y1 - 1980/3
N2 - T cells were obtained from mice immunized to keyhole limpet hemocyanin (KLH) and tested in culture for their proliferation to KLH or to concanavalin A. The T-cell proliferation to KLH depended on the presence of Ia-bearing macrophages. This was shown only after extensive manipulations of T cells depleting accessory cells by adherence to dishes and passage through nylon-wool columns. The macrophage dependency required homology with macrophages from strains of mice sharing the left side of H-2. The macrophage dependency was not bypassed by conditioned media from macrophages. In contrast, the proliferative response to concanavalin A, which was also macrophage dependent, showed no H-2 restriction and could be replaced by a macrophage-conditioned medium. Nonadherent spleen cells, extensively devoid of macrophages, could substitute for macrophages but at a hundredfold excess. Cells isolated in the fluorescence-activated cell sorter with anti-Ig reagents also showed an antigen-presenting function. We raise the question whether our results imply an antigen-presenting function of B cells.
AB - T cells were obtained from mice immunized to keyhole limpet hemocyanin (KLH) and tested in culture for their proliferation to KLH or to concanavalin A. The T-cell proliferation to KLH depended on the presence of Ia-bearing macrophages. This was shown only after extensive manipulations of T cells depleting accessory cells by adherence to dishes and passage through nylon-wool columns. The macrophage dependency required homology with macrophages from strains of mice sharing the left side of H-2. The macrophage dependency was not bypassed by conditioned media from macrophages. In contrast, the proliferative response to concanavalin A, which was also macrophage dependent, showed no H-2 restriction and could be replaced by a macrophage-conditioned medium. Nonadherent spleen cells, extensively devoid of macrophages, could substitute for macrophages but at a hundredfold excess. Cells isolated in the fluorescence-activated cell sorter with anti-Ig reagents also showed an antigen-presenting function. We raise the question whether our results imply an antigen-presenting function of B cells.
UR - http://www.scopus.com/inward/record.url?scp=0018870261&partnerID=8YFLogxK
U2 - 10.1016/0090-1229(80)90055-0
DO - 10.1016/0090-1229(80)90055-0
M3 - Article
C2 - 6966200
AN - SCOPUS:0018870261
SN - 0090-1229
VL - 15
SP - 434
EP - 443
JO - Clinical Immunology and Immunopathology
JF - Clinical Immunology and Immunopathology
IS - 3
ER -