TY - JOUR
T1 - Accelerating the Field of Epigenetic Histone Modification through Mass Spectrometry–Based Approaches
AU - Lu, Congcong
AU - Coradin, Mariel
AU - Porter, Elizabeth G.
AU - Garcia, Benjamin A.
N1 - Funding Information:
This work was supported by National Institutes of Health grants (AI118891 and CA196539) and a St Jude Consortium grant to B. A. G. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Publisher Copyright:
© 2021 THE AUTHORS.
PY - 2021
Y1 - 2021
N2 - Histone post-translational modifications (PTMs) are one of the main mechanisms of epigenetic regulation. Dysregulation of histone PTMs leads to many human diseases, such as cancer. Because of its high throughput, accuracy, and flexibility, mass spectrometry (MS) has emerged as a powerful tool in the epigenetic histone modification field, allowing the comprehensive and unbiased analysis of histone PTMs and chromatin-associated factors. Coupled with various techniques from molecular biology, biochemistry, chemical biology, and biophysics, MS has been used to characterize distinct aspects of histone PTMs in the epigenetic regulation of chromatin functions. In this review, we will describe advancements in the field of MS that have facilitated the analysis of histone PTMs and chromatin biology.
AB - Histone post-translational modifications (PTMs) are one of the main mechanisms of epigenetic regulation. Dysregulation of histone PTMs leads to many human diseases, such as cancer. Because of its high throughput, accuracy, and flexibility, mass spectrometry (MS) has emerged as a powerful tool in the epigenetic histone modification field, allowing the comprehensive and unbiased analysis of histone PTMs and chromatin-associated factors. Coupled with various techniques from molecular biology, biochemistry, chemical biology, and biophysics, MS has been used to characterize distinct aspects of histone PTMs in the epigenetic regulation of chromatin functions. In this review, we will describe advancements in the field of MS that have facilitated the analysis of histone PTMs and chromatin biology.
UR - http://www.scopus.com/inward/record.url?scp=85102690035&partnerID=8YFLogxK
U2 - 10.1074/MCP.R120.002257
DO - 10.1074/MCP.R120.002257
M3 - Article
C2 - 33203747
AN - SCOPUS:85102690035
SN - 1535-9476
VL - 20
JO - Molecular and Cellular Proteomics
JF - Molecular and Cellular Proteomics
M1 - 100006
ER -