TY - JOUR
T1 - Abundance and composition of indigenous bacterial communities in a multi-step biofiltration-based drinking water treatment plant
AU - Lautenschlager, Karin
AU - Hwang, Chiachi
AU - Ling, Fangqiong
AU - Liu, Wen Tso
AU - Boon, Nico
AU - Köster, Oliver
AU - Egli, Thomas
AU - Hammes, Frederik
N1 - Funding Information:
We would like to thank Aline Frossard for her support with the enzymatic assays, Sebastian Meylan and Jacqueline Traber for the liquid chromatography organic carbon detection (LC-OCD) measurements and analysis, and Dr. Lin Ye for help with using MOTHUR. We are grateful to the financial support of the EU project TECHNEAU (018320).
PY - 2014/10/1
Y1 - 2014/10/1
N2 - Indigenous bacterial communities are essential for biofiltration processes in drinking water treatment systems. In this study, we examined the microbial community composition and abundance of three different biofilter types (rapid sand, granular activated carbon, and slow sand filters) and their respective effluents in a full-scale, multi-step treatment plant (Zürich, CH). Detailed analysis of organic carbon degradation underpinned biodegradation as the primary function of the biofilter biomass. The biomass was present in concentrations ranging between 2-5×1015cells/m3 in all filters but was phylogenetically, enzymatically and metabolically diverse. Based on 16S rRNA gene-based 454 pyrosequencing analysis for microbial community composition, similar microbial taxa (predominantly Proteobacteria, Planctomycetes, Acidobacteria, Bacteriodetes, Nitrospira and Chloroflexi) were present in all biofilters and in their respective effluents, but the ratio of microbial taxa was different in each filter type. This change was also reflected in the cluster analysis, which revealed a change of 50-60% in microbial community composition between the different filter types.
AB - Indigenous bacterial communities are essential for biofiltration processes in drinking water treatment systems. In this study, we examined the microbial community composition and abundance of three different biofilter types (rapid sand, granular activated carbon, and slow sand filters) and their respective effluents in a full-scale, multi-step treatment plant (Zürich, CH). Detailed analysis of organic carbon degradation underpinned biodegradation as the primary function of the biofilter biomass. The biomass was present in concentrations ranging between 2-5×1015cells/m3 in all filters but was phylogenetically, enzymatically and metabolically diverse. Based on 16S rRNA gene-based 454 pyrosequencing analysis for microbial community composition, similar microbial taxa (predominantly Proteobacteria, Planctomycetes, Acidobacteria, Bacteriodetes, Nitrospira and Chloroflexi) were present in all biofilters and in their respective effluents, but the ratio of microbial taxa was different in each filter type. This change was also reflected in the cluster analysis, which revealed a change of 50-60% in microbial community composition between the different filter types.
KW - 454 pyrosequencing
KW - Adenosine tri-phosphate (ATP)
KW - Biofilter
KW - Drinking water
KW - Microbial communities
UR - http://www.scopus.com/inward/record.url?scp=84902356366&partnerID=8YFLogxK
U2 - 10.1016/j.watres.2014.05.035
DO - 10.1016/j.watres.2014.05.035
M3 - Article
C2 - 24937356
AN - SCOPUS:84902356366
SN - 0043-1354
VL - 62
SP - 40
EP - 52
JO - Water Research
JF - Water Research
ER -