Absence of cytokine receptor-dependent specificity in red blood cell differentiation in vivo

Mark A. Goldsmith, Aki Mikami, Yun You, Kathleen D. Liu, Liza Thomas, Pamela Pharr, Gregory D. Longmore

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Erythropoietin (EPO) is required for red blood cell development, but whether EPO-specific signals directly instruct erythroid differentiation is unknown. We used a dominant system in which constitutively active variants of the EPO receptor were introduced into erythroid progenitors in mice. Chimeric receptors were constructed by replacing the cytoplasmic tail of constitutively active variants of the EPO receptor with tails of diverse cytokine receptors. Receptors linked to granulocyte or platelet production supported complete erythroid development in vitro and in vivo, as did the growth hormone receptor, a nonhematopoietic receptor. Therefore, EPOR- specific signals are not required for terminal differentiation of erythrocytes. Furthermore, we found that cellular context can influence cytokine receptor signaling.

Original languageEnglish
Pages (from-to)7006-7011
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number12
DOIs
StatePublished - Jun 9 1998

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