TY - JOUR
T1 - A yeast actin-related protein homologous to that in vertebrate dynactin complex is important for spindle orientation and nuclear migration
AU - Muhua, Li
AU - Karpova, Tatiana S.
AU - Cooper, John A.
N1 - Funding Information:
We aregrateful to Christopher Hug, Mark Johnston, LindaRiles, Dorothy Schafer, and Jim Waddle for advice and assistance. Kelly Tatchell kindly informed us of the nuclear migration defect in the capping protein mutants, David Helfman provided actin-RPV cDNA and antibodies, Ulrike Wintersberger shared the ACT3 gene before publication, and Elaine Yeh and Kerry Bloom provided DHCl strains and plasmids. This work was supported by a grant from the National Institutes of Health (GM47337). J. A. is an Established Investigator of the American Heart Association.
PY - 1994/8/26
Y1 - 1994/8/26
N2 - Spindle orientation controls nuclear migration and segregation during mitosis. In yeast, defects in dynein and astral microtubules lead to abnormal spindle orientation and nuclear migration. Dynactin complex is necessary for dynein-mediated vesicle motility in vitro. The major polypeptide of dynactin complex is an actin-related protein in the family Arpl. We have identified in S. cerevisiae a novel actin-related gene, ACT5, In the Arpi family. An act5 null mutant has defects in spindle orientation and nuclear migration, as does overexpression of Act5p. The phenotype of a double mutant lacking dynein and Act5p is similar to that of single mutants. Therefore, dynactin complex is in the same pathway as dynein and may be necessary for the action of dynein in vivo.
AB - Spindle orientation controls nuclear migration and segregation during mitosis. In yeast, defects in dynein and astral microtubules lead to abnormal spindle orientation and nuclear migration. Dynactin complex is necessary for dynein-mediated vesicle motility in vitro. The major polypeptide of dynactin complex is an actin-related protein in the family Arpl. We have identified in S. cerevisiae a novel actin-related gene, ACT5, In the Arpi family. An act5 null mutant has defects in spindle orientation and nuclear migration, as does overexpression of Act5p. The phenotype of a double mutant lacking dynein and Act5p is similar to that of single mutants. Therefore, dynactin complex is in the same pathway as dynein and may be necessary for the action of dynein in vivo.
UR - http://www.scopus.com/inward/record.url?scp=0028025571&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(94)90531-2
DO - 10.1016/0092-8674(94)90531-2
M3 - Article
C2 - 8069915
AN - SCOPUS:0028025571
SN - 0092-8674
VL - 78
SP - 669
EP - 679
JO - Cell
JF - Cell
IS - 4
ER -