A xylosylphosphotransferase of Cryptococcus neoformans acts in protein O-glycan synthesis

Morgann C. Reilly, Kazuhiro Aoki, Zhuo A. Wang, Michael L. Skowyra, Matthew Williams, Michael Tiemeyer, Tamara L. Doering

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Cryptococcal meningoencephalitis is an AIDS-defining illness caused by the opportunistic pathogen Cryptococcus neoformans. This organism possesses an elaborate polysaccharide capsule that is unique among pathogenic fungi, and the glycobiology of C. neoformans has been a focus of research in the field. The capsule and other cellular glycans and glycoconjugates have been described, but the machinery responsible for their synthesis remains largely unexplored. We recently discovered Xpt1p, an enzyme with the unexpected activity of generating a xylosephosphate-mannose linkage. We now demonstrate that this novel activity is conserved throughout the C. neoformans species complex, localized to the Golgi apparatus, and functions in the O-glycosylation of proteins. We also present the first survey of O-glycans from C. neoformans.

Original languageEnglish
Pages (from-to)26888-26899
Number of pages12
JournalJournal of Biological Chemistry
Volume286
Issue number30
DOIs
StatePublished - Jul 29 2011

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