A weak upstream promoter gives rise to long human β-globin RNA molecules

Timothy J. Ley, Arthur W. Nienhuis

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The 5′ ends of most normal human β globin mRNA molecules correspond to a single transcription initiation site, often referred to as the "CAP" site (1-4). Using S1 nuclease mapping and primer extension techniques, we have determined that a minority of β globin gene transcripts are longer at the 5′ ends. These longer molecules comprise about 10% of total β globin RNA molecules in normal human bone marrow cells and in peripheral blood reticulocytes. The long molecules are transcribed only from the sense strand of DNA and are probably spliced correctly. A DNA segment that includes imperfect "CCAAT" and "TATA" promoter-like sequences begins approximately 150 base pairs (bp) upstream from the normal β globin gene promoter; this "pseudo-promoter" may function in the initiation of the long globin RNA molecules.

Original languageEnglish
Pages (from-to)1041-1048
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume112
Issue number3
DOIs
StatePublished - May 16 1983
Externally publishedYes

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