TY - JOUR
T1 - A unique cardiac cytosolic acyltransferase with preferential selectivity for fatty acids that form cyclooxygenase/lipoxygenase metabolites and reverse essential fatty acid deficiency
AU - Needleman, Philip
AU - Wyche, Angela
AU - Sprecher, Howard
AU - Elliott, William J.
AU - Evers, Alex
N1 - Funding Information:
Supported by NIH grant HL 17646 (SCOR), the authors appreciate helpful suggestions from Richard Gross.
PY - 1985/9/11
Y1 - 1985/9/11
N2 - The rabbit heart contains a cytosolic enzyme which selectively incorporates polyunsaturated fatty acids into phosphatidylcholine. This unique acyltransferase is selective for fatty acids, thus far tested, that are substrates for cyclooxygenase or lipoxygenase (i.e., arachidonic, eicosapentaenoic, linoleic and dihomo-γ-linoleic acids) or which reverse the symptoms of essential fatty acid deficiency (columbinic acid). On the other hand, palmitic, oleic, 5,8,11-eicosatrienoic (n - 9, Mead acid), and docosatetraenoic acid (n - 6, adrenic acid) were not incorporated in phospholipids by the cytosolic acyltransferase. No such fatty acid selectivity was exhibited by the cytosolic acyl-CoA synthetase or by the acyltransferase activities present in cardiac microsomes and mitochondria.
AB - The rabbit heart contains a cytosolic enzyme which selectively incorporates polyunsaturated fatty acids into phosphatidylcholine. This unique acyltransferase is selective for fatty acids, thus far tested, that are substrates for cyclooxygenase or lipoxygenase (i.e., arachidonic, eicosapentaenoic, linoleic and dihomo-γ-linoleic acids) or which reverse the symptoms of essential fatty acid deficiency (columbinic acid). On the other hand, palmitic, oleic, 5,8,11-eicosatrienoic (n - 9, Mead acid), and docosatetraenoic acid (n - 6, adrenic acid) were not incorporated in phospholipids by the cytosolic acyltransferase. No such fatty acid selectivity was exhibited by the cytosolic acyl-CoA synthetase or by the acyltransferase activities present in cardiac microsomes and mitochondria.
KW - (Rabbit heart)
KW - Acyl transferase
KW - Acyl-CoA synthetase
KW - Fatty acid
KW - Phospholipid
UR - http://www.scopus.com/inward/record.url?scp=0022405837&partnerID=8YFLogxK
U2 - 10.1016/0005-2760(85)90075-X
DO - 10.1016/0005-2760(85)90075-X
M3 - Article
C2 - 3927984
AN - SCOPUS:0022405837
VL - 836
SP - 267
EP - 273
JO - Biochimica et Biophysica Acta - Lipids and Lipid Metabolism
JF - Biochimica et Biophysica Acta - Lipids and Lipid Metabolism
SN - 0005-2760
IS - 2
ER -